Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Neutrophil-to-lymphocyte ratio is associated with diabetic peripheral neuropathy in type 2 diabetes patients.

Diabetes research and clinical practice·2017
Same author

Genetic variation and phylogeographic structure of the cotton aphid, Aphis gossypii, based on mitochondrial DNA and microsatellite markers.

Scientific reports·2017
Same author

Tetramethylpyrazine blocks TFAM degradation and up-regulates mitochondrial DNA copy number by interacting with TFAM.

Bioscience reports·2017
Same author

Mitochondrial LON protease-dependent degradation of cytochrome c oxidase subunits under hypoxia and myocardial ischemia.

Biochimica et biophysica acta. Bioenergetics·2017
Same author

The efficacy and safety of epinephrine for postoperative bleeding in total joint arthroplasty: A PRISMA-compliant meta-analysis.

Medicine·2017
Same author

The correlation between histological gastritis staging- 'OLGA/OLGIM' and serum pepsinogen test in assessment of gastric atrophy/intestinal metaplasia in China.

Scandinavian journal of gastroenterology·2017

相关实验视频

Updated: Jun 12, 2025

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

10.8K

想象神经元膜胆固醇与分裂光蛋白标记为YDQA传感器的胆固醇.

Yi Xu1, Saixuan Li1, Yiran Xu1

  • 1State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, PR China.

Journal of lipid research
|March 21, 2025
PubMed
概括

研究人员开发了一种新型传感器,可以在体内可视化神经元胆固醇. 这种工具在阿尔茨海默病模型中揭示了明显的胆固醇变化,为神经系统疾病提供了新的见解.

关键词:
阿尔茨海默氏症是阿尔茨海默氏症的一种疾病.胆固醇 胆固醇 胆固醇 是一种血膜是一种等离子体膜.传感器 传感器 传感器

更多相关视频

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
12:58

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

Published on: November 20, 2012

13.1K
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
08:31

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Published on: July 29, 2021

1.5K

相关实验视频

Last Updated: Jun 12, 2025

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

10.8K
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
12:58

Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons

Published on: November 20, 2012

13.1K
Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
08:31

Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6

Published on: July 29, 2021

1.5K

科学领域:

  • 神经科学是一个神经科学.
  • 生物化学 生化学
  • 细胞生物学 细胞生物学

背景情况:

  • 胆固醇对神经元等离子体膜 (PM) 功能和大脑健康至关重要.
  • 破坏胆固醇平衡与阿尔茨海默氏症 (AD) 等神经系统疾病有关.
  • 对神经元胆固醇的体内可视化仍然是理解AD病理学的重大挑战.

研究的目的:

  • 开发和验证一种用于检测神经元PM胆固醇的新型传感器.
  • 为了研究AD小鼠模型中的胆固醇分布变化.

主要方法:

  • 基于分离光蛋白的传感器 (sfPMcho) 的生成,使用胆固醇依赖性细胞酶衍生物 (YDQA).
  • 在细胞和C. elegans模型中验证sfPMcho传感器.
  • 在AD的5X FAD和APOE KO小鼠模型中应用sfPMcho传感器.

主要成果:

  • sfPMcho传感器在体内有效检测神经元中的PM胆固醇.
  • 在AD小鼠模型中观察到胆固醇分布的显著变化.
  • 具体来说,PM胆固醇稀少,在神经元体中聚集,但在神经纤维中积累.

结论:

  • 该sfPMcho传感器为体内检测神经元PM胆固醇提供了一个有价值的新工具.
  • 这项研究揭示了AD相关病理中的细胞水平的特定胆固醇异常.
  • 预计这种传感器的进一步开发将推动AD研究.