相关实验视频

Updated: May 21, 2025

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

基因性质是不同扰动的转录变异的基础

Saburo Tsuru¹, Chikara Furusawa^2,3,4

¹Universal Biology Institute, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. saburotsuru@gmail.com.

Nature communications

|March 22, 2025

概括

基因调节网络通过引导遗传和环境影响来塑造表型变异. 这项研究揭示了特定基因属性如何解释转录变异的差异,影响进化潜力.

科学领域:

进化生物学是进化的生物学.
系统生物学系统生物学
遗传学遗传学是一个

背景情况:

现型进化依赖于从遗传或环境因素变异的可用性.
众所周知,基因调节网络 (GRNs) 影响表型变异.
由于GRN的形状变化,其确切的机制仍然在很大程度上是未知的.

研究的目的:

研究基因调控网络如何影响表型变异.
在各种干扰下对大肠杆菌的转录变异进行表征.
为了确定与基因特异性转录变异相关的遗传性质.

主要方法:

对大肠杆菌的转录变异的分析.
扰乱实验 (遗传和环境).
遗传性质与转录变异模式的相关性.

主要成果:

基因调节网络在将遗传和环境影响引导到表型变异方面发挥着至关重要的作用.
特定的遗传性质与转录变异性中的基因对基因差异相关.
跨扰乱的表型变异性的共享模式是由GRNs塑造的.

结论:

GRN是表型变异的关键调节者.
了解GRN架构对于预测进化轨迹至关重要.
这项研究提供了关于GRN如何调节表型多样性的产生机制的见解.

更多相关视频

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Published on: September 13, 2018

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

相关概念视频

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Structure of a Gene

Structure of a Gene

A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.