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高质量的染色体层次的基因组组合Dracocephalum rupestre Hance的高质量染色体层次的基因组组合

Qian Zhao^1,2, Zixu Fan¹, Hui Yu^3,4

¹Inner Mongolia Key Laboratory of Disease-Related Biomarkers, The Second Affiliated Hospital, Baotou Medical College, Baotou, 014030, China.

Scientific data

|March 22, 2025

概括

创建了一个高质量的基因组组装Dracocephalum rupestre,一个传统的中国草药. 此资源有助于研究其药用特性和遗传基础.

科学领域:

基因组学就是基因组学.
植物科学植物科学
药理学药理学是指药理学的学科.

背景情况:

Dracocephalum rupestre Hance是一种来自Labiatae家族的传统中国草药.
它具有抗炎症,抗病毒和抗瘤的特性.
缺乏Dracocephalum属的参考基因组,阻碍了对D. rupestre的繁殖,栽培和药用潜力的研究.

研究的目的:

为了生成一个高质量的,染色体水平的基因组组装Dracocephalum rupestre.
为功能性基因组学和D. rupestre.的繁殖研究提供必要的基因组资源.

主要方法:

使用Pacbio HiFi测序进行初始基因组装.
采用了Hi-C支架技术来实现染色体水平的整合.
用于基因预测和功能注释的生物信息分析.

主要成果:

实现了435.45 Mb的基因组组合,连接N50为49.83 Mb,支架N50为59.06 Mb.
该基因组成功定在7个染色体上,整合效率为96.96%.
预测了25865个编码蛋白质的基因,其中98.23%的功能注释.

结论:

D. rupestre的染色体级基因组组合为基因组研究提供了宝贵的资源.
有助于理解D. rupestre的药用特性和独特特征的遗传基础.
允许未来的功能基因组学研究和这一重要的药用草本的育种计划.

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