通过低碳微生物制造重新编程酵母新陈代谢,以定制富含粉的微粒
Zhihui Shi1,2,3,4,5, Zhaoyu Xu1,4, Weihe Rong1,3,4,5
1Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
Nature communications
|March 22, 2025
概括
微生物制造使用二氧化碳合成的酸盐制造富含粉的酵母. 这种低碳,土地独立的方法实现了高粉生产率,超过了作物种植和其他微生物系统.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 粉是重要的食品成分和工业材料.
- 目前的粉生产依赖于农业,需要耕地和资源.
- 低碳微生物制造提供了一个可持续的,独立于土地的替代方案.
研究的目的:
- 用低碳原料设计油性酵母,以高效生产粉.
- 为了提高粉产量和生产力,超越传统方法.
- 为微生物粉合成建立一个可定制的平台.
主要方法:
- 在酵母中重新连接粉生物合成和葡萄糖生成途径.
- 调节酵母细胞形态,以形成微粒.
- 使用由二氧化碳衍生的电合成乙酸作为基质.
主要成果:
- 工程酵母积累了粉,占干细胞重量的47.18%.
- 实现了243.7g/m2/d的空间时间粉生产率,比作物种植高50倍.
- 已证明体积生产率为160.83mg/L/h,比其他微生物系统高出一个数量级.
- 展示了可调节的粉成分和粉与蛋白质的比例.
结论:
- 工程酵母有效地将二氧化碳衍生的酸盐转化为高产粉.
- 这种微生物平台为粉制造提供了卓越的生产力和定制性.
- 该方法促进了低碳营养制造和生物质转化.
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