Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA Editing02:23

RNA Editing

8.8K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.4K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

23.1K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
23.1K
RNA Stability01:53

RNA Stability

33.1K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.1K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.9K
RNA Splicing01:32

RNA Splicing

55.9K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structure of the NAT10 acetyltransferase and mechanism of tRNA acetylation.

Nature communications·2026
Same author

A lipid cue drives the subcellular localization of a self-inserting bacterial transmembrane protein.

bioRxiv : the preprint server for biology·2026
Same author

Hierarchical small molecule inhibition of MYST acetyltransferases.

Nature communications·2026
Same author

Archaeal family B DNA polymerase facilitates lagging strand DNA replication in the Thermococcales.

Nucleic acids research·2026
Same author

The LARP1 RRM functions as a ribosome responsive regulator of TOP mRNAs.

bioRxiv : the preprint server for biology·2026
Same author

Rerouting reductant flux via protein tethering enhances biohydrogen production in Thermococcus kodakarensis.

Applied microbiology and biotechnology·2026
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
查看所有相关文章

相关实验视频

Updated: May 21, 2025

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.2K

一个特定序列的RNA乙化催化剂.

Supuni Thalalla Gamage1, Shereen Howpay Manage1, Aldema Sas-Chen2

  • 1Chemical Biology Laboratory, National Cancer Institute, Frederick, MD 21702, United States.

Nucleic acids research
|March 22, 2025
PubMed
概括
此摘要是机器生成的。

这项研究描述了Thermococcus kodakarensis Nat10 (TkNat10),这是一个对古人类耐热性至关重要的酶. TkNat10在高温下修改RNA,为RNA乙化提供了洞察力,也是研究N4-乙基丁 (ac4C) 功能的工具.

更多相关视频

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

3.9K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.5K

相关实验视频

Last Updated: May 21, 2025

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

7.2K
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
07:26

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Published on: December 26, 2020

3.9K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.5K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 考古研究 考古研究

背景情况:

  • N4-乙基丁 (ac4C) 是一种重要的RNA修饰.
  • 赛蒂丁乙转移酶负责ac4C的结合.
  • 古代生物拥有独特的耐热机制.

研究的目的:

  • 在生物化学上描述Thermococcus kodakarensis Nat10 (TkNat10).
  • 为了研究TkNat10在古人类耐热性中的作用.
  • 探索TkNat10的基质特异性和辅因子要求.

主要方法:

  • 生物化学测试以确定TkNat10活性.
  • 转录组全方位分析以识别修饰的RNA.
  • 高通量突变发生来定义基质识别决定因素.
  • 酶工程是为了改变辅因子的特异性.

主要成果:

  • 对于T. kodakarensis在高温条件下保持健康,TkNat10活动是必不可少的.
  • TkNat10表现出强大的独立活性,取决于温度,ATP和乙-CoA.
  • TkNat10优先修改具有5'-CCG-3'共识序列的非结构化RNA.
  • TkNat10可以被设计为利用propionyl-CoA.

结论:

  • TkNat10是古代RNA乙化和耐热性中的关键酶.
  • 了解TkNat10为考古RNA修饰研究提供了一个框架.
  • TkNat10是分析ac4C-依赖的RNA-蛋白相互作用的宝贵工具.