Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

579
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
579
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

204
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
204

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

NMR of Fully and Partially <sup>13</sup>C-Enriched Biomass Enhances Pendent Group Structural Characterization.

Analytical chemistry·2026
Same author

Protonated Structure of EmrE Reveals C-terminal Tail Gating Mechanism.

bioRxiv : the preprint server for biology·2026
Same author

Breakdown products of the fungicide Fludioxonil may account for observed environmental impact: potential implications for human health.

PeerJ·2026
Same author

Intrinsic conformational equilibria position arrestin-2 for activation.

Protein science : a publication of the Protein Society·2026
Same author

Fast folding of anthrax lethal factor N-terminal domain independent of proline isomerization.

Biophysical journal·2026
Same author

Proton hopping and secondary gating reveal a universal transport mechanism in a minimal bacterial transporter EmrE.

Biophysical journal·2026
Same journal

Efficient Syngas Photoproduction Enabled by Electronic Engineering of Co-Immobilized Imine COFs.

Angewandte Chemie (International ed. in English)·2026
Same journal

Pathway Controlled Phase Separation of Minimal Building Blocks Utilizing a Dissociative Chemical Transformation.

Angewandte Chemie (International ed. in English)·2026
Same journal

Interaction Hierarchy and Polymorphic Structure-Property Dynamics in Luminescent Molecular Crystals.

Angewandte Chemie (International ed. in English)·2026
Same journal

The Role of Zn-Hf Site Proximity and Oxygen Vacancies for Methanol Formation Over ZnHfO<sub>x</sub> Catalysts Under CO<sub>2</sub> Hydrogenation Conditions.

Angewandte Chemie (International ed. in English)·2026
Same journal

Breaking the Linear Scaling Relationship: Bioinspired Electronic Coupling in S-Bridged Fe-Fe Dual Sites for Efficient Oxygen Reduction.

Angewandte Chemie (International ed. in English)·2026
Same journal

Programming Bio-Bio Electronic Interfaces for Light-Driven Interspecies Electron Transfer.

Angewandte Chemie (International ed. in English)·2026
查看所有相关文章

相关实验视频

Updated: May 21, 2025

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.6K

在现场以光驱动的pH调制用于NMR研究.

Aarav Barde1, Ruixian Han2, Martin A Olson1

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, 53706, USA.

Angewandte Chemie (International ed. in English)
|March 22, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种使用光激活光酸控制核磁共振 (NMR) 样本pH值的新方法. 这种技术允许在NMR磁铁内进行精确的,非侵入性的pH调整,减少样品处理并提高实验可靠性.

关键词:
缓冲区是一个缓冲区.这是NMR的NMR.摄影照明的照明方式光酸光酸是一种光酸.质子突变的发生.定位 定位 定位 定位 定位

更多相关视频

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
16:19

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

11.7K
Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
09:46

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

Published on: April 28, 2014

14.9K

相关实验视频

Last Updated: May 21, 2025

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
10:54

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

10.6K
Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
16:19

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

Published on: September 10, 2013

11.7K
Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
09:46

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy

Published on: April 28, 2014

14.9K

科学领域:

  • 化学 化学 化学
  • 生物物理学的生物物理.
  • 分析化学 分析化学

背景情况:

  • 质子交换在化学和生物系统中至关重要.
  • 核磁共振 (NMR) 光谱为研究质子事件提供了特定位置的分辨率.
  • 传统的NMRpH定位需要大量的样本处理,并且可能导致样本丢失.

研究的目的:

  • 引入一种新的,非侵入性的方法来控制NMR样本中的pH值.
  • 用光激活光酸来证明精确的,在现场的pH调制.
  • 减少样品处理,提高pH依赖的NMR研究的可靠性.

主要方法:

  • 使用水溶性光酸在光照照明时改变溶液pH值.
  • 通过调整照明波长和强度来控制pH值.
  • 使用内部标准在现场监测pH值,使用pH值敏感的化学变化.
  • 将该方法应用于具有显著缓冲能力 (> 100μM) 的蛋白质样本.

主要成果:

  • 在NMR磁铁中实现了精确和校准的pH控制.
  • 从近中性到酸性条件的pH值成功改变.
  • 展示了足够的质子的释放,以诱导缓冲样本的pH值有意义的变化.
  • 显著减少了对广泛样本操纵的需求.

结论:

  • 光诱导的pH控制为NMR实验提供了一个强大而可靠的替代方案.
  • 这种新的方法提高了依赖pH的NMR研究的效率和准确性.
  • 该方法具有广泛的适用性,用于研究各种生物和化学样本中的质子化事件.