一个DOF转录因子GLW9/OsDOF25调节米的谷粒形状和角
Huan Shi1,2, Pingbo Li3, Peng Yun4
1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, China.
Plant biotechnology journal
|March 22, 2025
概括
研究人员确定了GLW9基因,这是一种控制米粒形状和机角度的转录因子. 这一发现为改善大米产量和质量提供了新的遗传资源.
科学领域:
- 植物遗传学 植物遗传学
- 农业学是一种农业学.
- 分子生物学分子生物学
背景情况:
- 谷粒形状和耕角度是影响大米产量和质量的关键农学特征.
- 了解这些特征的遗传调节对于作物改进至关重要.
研究的目的:
- 为绘制克隆和描述基因GLW9 (9.染色体上的谷物长度和宽度) 的功能.
- 阐明GLW9调节谷粒形状和轮角度的分子机制.
主要方法:
- 基于地图的克隆被用来识别GLW9基因.
- 进行了分子分析以确定GLW9的功能和监管目标.
- 进行了基因表达和促进体结合试验.
主要成果:
- GLW9编码了DOF家族的转录因子 (OsDOF25),该转录因子正调节谷粒长度,负调节谷粒宽度.
- GLW9上调EXPA6的表达,促进细胞扩张和影响谷物形状.
- GLW9直接调节上升OsPIN1b的表达,从而减少了杆角度.
结论:
- 在大米中,GLW9充当关键调节器,协调谷粒形状和角.
- 这项研究为改善大米农学特征提供了宝贵的基因资源和理论见解.
更多相关视频
05:22Transverse Sectioning of Mature Rice Oryza sativa L. Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
Published on: January 25, 2022
3.6K
10:29Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
6.4K
相关概念视频
Master Transcription Regulators
6.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.8K
General Transcription Factors
5.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.1K
Transcription Elongation Factors
3.3K
3.3K
Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
Regulation of Expression at Multiple Steps
862
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
862
Transgenic Plants
7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K
