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在雅克中,SETD2通过基素甲基化和母体mRNA降解来调节体外卵细胞的成熟

Hui Zhang¹, Yangyang Pan¹, Meng Wang¹

¹College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, China; Gansu Provincial Livestock Embryo Engineering Technology Innovation Center, Lanzhou, 730070, China.

|March 22, 2025

概括

含有SET域2 (SETD2) 的海斯甲基转移酶对雅克卵细胞成熟至关重要. 抑制SETD2会损害组织蛋白甲基化,降低成熟率,并影响辅助生殖技术中的关键蛋白质表达.

科学领域:

生殖生物学生殖生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
动物育种动物育种

背景情况:

试管成熟 (IVM) 是辅助生殖技术 (ART) 的关键.
雅克卵细胞IVM研究是有限的,阻碍了雅克繁殖应用.
了解雅克卵细胞成熟的分子机制至关重要.

研究的目的:

调查雅克卵细胞IVM期间的转录组变化.
为了确定雅克卵细胞成熟的关键调节者.
阐明基因组甲基转移酶含SET域2 (SETD2) 在蛋细胞成熟中的作用.

主要方法:

不成熟和成熟的雅克卵细胞的高通量mRNA测序.
转录组分析以确定关键的监管因素.
在卵细胞成熟过程中使用EZM0414对SETD2进行药理抑制.
评估基因素甲基化,卵细胞成熟率和基因表达.

主要成果:

SETD2被确定为雅克卵细胞成熟的关键因素.
抑制SETD2降低了基因素甲基化和体外成熟率.
抑制SETD2导致母体mRNA降解受损,并减少关键蛋白的表达 (TSG6,GDF9,PTGS2).
卵细胞分泌和累积膨胀与SETD2活性呈正相关性.

结论:

在雅克卵细胞成熟过程中,SETD2起着至关重要的作用.
在SETD2中,SETD2通过组织素甲基化和母体mRNA降解来调节卵细胞成熟.
在雅克中,SETD2对于维持卵细胞分泌和累积膨胀至关重要.

关键词:

H3K36me3是什么意思 H3K36me3是什么意思卵细胞成熟的过程这就是SETD2的原因.雅克雅克雅克雅克是什么意思

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