采样方法对收获前商业肉生产中的沙门氏菌检测和量化有影响
Emily E Cason1, Marco Reina1, David Ayala-Velasteguí1
1Poultry Diagnostic and Research Center, University of Georgia, Athens, Georgia.
Poultry science
|March 22, 2025
概括
准确的收获前沙门氏菌监测在 brojlers 是至关重要的. 靴和靴滚筒是检测肉群中的沙门氏菌流行情况的最可复制和最可靠的采样方法.
科学领域:
- 兽医微生物学 兽医微生物学
- 食品安全 食品安全
- 禽畜科学 禽畜科学 禽畜科学
背景情况:
- 有效的收获前沙门氏菌监测对于肉生产至关重要.
- 准确,可靠和可重复的采样方法是需要的,以评估沙门氏菌.
- 目前的方法在检测沙门氏菌流行率方面的有效性各不相同.
研究的目的:
- 评估和比较六种不同的采样方法来检测肉中的沙门氏菌.
- 确定采摘前沙门氏菌监测的最准确,可靠和可重复的方法.
- 在代实验中评估沙门氏菌的流行和量化.
主要方法:
- 测试了六种采样方法:靴子,静电卷机,羽毛擦拭器,水擦拭器,便抓取和垃圾抓取.
- 患病率通过定量聚合酶链反应 (qPCR) 和培养方法确定.
- 通过代实验和统计分析 (费舍尔的精确测试,麦克纳马的测试,F-测试,线性混合效应模型) 来评估可重复性和可靠性.
主要成果:
- 靴和靴滚筒被确定为最可复制的采样方法.
- 与其他方法相比,这两种方法在检测沙门氏菌发病率方面表现出很高的准确性.
- 农场和房子对观察到的沙门氏菌检测差异作出了重大贡献.
结论:
- 采样方法显著影响沙门氏菌检测和肉的负载恢复.
- 由于其可再生性和可靠性,建议在收获前对沙门氏菌进行监测.
- 这些方法提供了最可靠的结果,以表明在生产期间肉群中沙门氏菌的流行率.
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