通过肠道上皮质运输取决于电压通通道和内分泌系统
Nithipak Thammayon1, Kannikar Wongdee2, Jarinthorn Teerapornpuntakit3
1Center of Calcium and Bone Research (COCAB), Faculty of Science, Mahidol University, Bangkok, Thailand; Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand; Graduate Program in Molecular Medicine, Faculty of Science, Mahidol University, Bangkok, Thailand.
Biochemical and biophysical research communications
|March 22, 2025
概括
肠道神经元通过电压通道 (Nav) 和CB1受体显著影响肠道的吸收. 阻断Nav通道的四毒素 (TTX) 减少了的运输,突出了这个过程的神经调节.
科学领域:
- 胃肠病学 胃肠病学
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 肠道的吸收主要由像维生素D这样的激素调节.
- 肠道神经元在调节肠道运输中的作用仍然在很大程度上未被探索.
- 电压通道 (Nav) 存在于肠神经元中,可能会影响它们的功能.
研究的目的:
- 研究肠道神经元中Nav通道对肠道运输的贡献.
- 为了确定神经因子是否可以调节跨体运输.
- 探索Nav通道,CB1受体和吸收之间的相互作用.
主要方法:
- 在基分子对接中预测TTX与Nav通道的结合.
- 使用放射性Ca的室内实验来测量大鼠的十二指甲状腺运输.
- 使用Caco-2细胞单层的实验,以评估在没有Nav表达细胞的情况下,TTX对运输的影响.
- 用CB1激动剂和一氧化碳释放分子 (CORM-2) 给药,以评估它们对运输的影响.
主要成果:
- 分子对接表明TTX可以封闭Nav通道孔.
- 甲毒素 (TTX) 显著降低了小鼠十二指肠中白诱导的运输,但没有降低Caco-2细胞的运输.
- TTX并没有影响十二指甲膜对Na+或Cl-的透性,也没有影响横细胞的抗性.
- 一个CB1激动剂增强了十二指肠运输,这一效应被TTX减弱.
- CORM-2对十二指肠运输没有影响.
结论:
- 肠道神经元中的电压通道 (Nav) 在调节肠道吸收方面发挥作用.
- CB1受体信号传递也有助于十二指肠运输.
- 这些发现支持控制肠道吸收的神经控制机制的存在.
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