Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Large-Scale Cohort Study on the Genetic and Phenotypic Findings of Palmoplantar Keratodermas in the Chinese Population.

Experimental dermatology·2026

Same author

ChSCL9 negatively regulates citric acid accumulation via repressing PH4-PH5 module in kumquat.

Plant physiology·2026

Same author

Trigger-day hCG effects on DNA methylation and neurodevelopment in ART offspring.

Nature communications·2026

Same author

Establishment of a Genetic Transformation System for <i>Hippophae gyantsensis</i> and the Regulatory Role of <i>Hgfw2.2</i> and <i>Hgfw3.2</i> in Fruit Size.

Plants (Basel, Switzerland)·2026

Same author

The transcription factor LSD-one-like 2 binds to a novel identified cis-element LBE (GCCAAAAC) and regulates molecular response of Populus davidiana×P. bolleana to salt stress.

International journal of biological macromolecules·2026

Same author

Clinical efficacy and safety of an overall strategy of immunotherapy combined with chemoradiotherapy for limited-stage small cell lung cancer (including induction ± consolidation ± second-line immunotherapy): A propensity score matching study based on real-world data.

Cancer treatment and research communications·2026

Search research articles

相关实验视频

Updated: May 21, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

高吞吐量分子记录的克隆和验证系统.

Anqi Zhao¹, Michelle M Chan¹

¹Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, United States; Department of Molecular Biology, Princeton University, Princeton, NJ, United States.

Methods in enzymology

|March 22, 2025

概括

此摘要是机器生成的。

这项研究详细介绍了基于CRISPR-Cas9的分子记录器,用于高分辨率的血统追踪. 优化的系统可以在发育和疾病期间精确追踪细胞史.

关键词:

克里斯普尔-Cas9是什么意思细胞工程细胞工程基因组编辑基因组编辑谱系追踪谱系追踪谱系追踪分子记录器分子记录器

更多相关视频

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

相关实验视频

Last Updated: May 21, 2025

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物技术是生物技术.

背景情况:

分子记录技术捕获细胞历史.
血统追踪对于理解发育,干细胞维护和瘤进化至关重要.
现有的方法可能缺乏复杂生物过程所需的分辨率.

研究的目的:

描述构建基于CRISPR-Cas9的分子记录器系统用于谱系追踪的优化程序.
为了验证这种新技术的性能,用于高分辨率的细胞历史记录.
为技术设计,克隆,细胞系生成和实验评估提供指导.

主要方法:

使用CRISPR-Cas9条码编辑来引入基因突变的工程DNA录音带.
基因组集成DNA录音带的稳定记录.
通过单细胞RNA测序进行读取.
开发优化的克隆和验证协议.

主要成果:

成功构建了一个分子记录器谱系追踪系统.
高分辨率的血统树重建的演示.
优化克隆和细胞系生成程序的验证.
通过时间过程实验评估系统性能.

结论:

描述的分子记录器技术为高分辨率的血统追踪提供了一个强大的工具.
优化的程序促进了该系统的构建和应用.
这项技术对研究哺乳动物发育,干细胞生物学和癌症进化有着广泛的影响.