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从肠道细菌Cronobacter malonaticus PO3分泌的三性皮促进了结直肠癌的发生
Rajesh Padumane Shastry1, Asif Hameed2, Shukla Banerjee3
1Division of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangaluru, 575018, India. rpshastry@yenepoya.edu.in.
Scientific reports
|March 23, 2025
概括
一种新的Cronobacter malonaticus菌株 (PO3) 和它的 (P506) 在临床前模型中被发现可以促进结直肠癌的生长和息肉的发展. 这表明这种细菌在人类结肠癌中具有潜在的致癌作用.
科学领域:
- 微生物学 微生物学
- 在瘤学瘤学.
- 胃肠病学 胃肠病学
背景情况:
- 克罗诺细菌物种是已知的病原体,通常与脑膜炎和死性肠球炎有关.
- 目前尚不清楚Cronobacter在人类结直肠癌 (CRC) 发病过程中的作用.
- 研究新型微生物对CRC的贡献对于了解疾病发展至关重要.
研究的目的:
- 为了调查克罗诺细菌在人类结直肠癌中的潜在参与.
- 从结肠癌患者的便样本中分离和描述一种新型的Cronobacter菌株.
- 确定孤立菌株及其分泌成分对CRC进展的影响.
主要方法:
- 从结肠癌患者的便样本中分离和描述一种新型的Cronobacter malonaticus菌株 (PO3).
- 实验室试验评估PO3分泌体对结直肠癌细胞增殖的影响 (活力,Ki-67表达).
- 高分辨率质谱 (HRMS) 用于识别分泌体中的新;使用BALB/c小鼠模型进行体内研究,以评估分泌体和识别的 (P506) 对聚发育的影响.
主要成果:
- 新的C. malonaticus PO3菌株被隔离和表征.
- 在试验室中,PO3分泌体显著增强了结直肠癌细胞的增殖,而没有诱导细胞死亡.
- 在PO3分泌体中发现的一种新型上,P506,剂量依赖地促进了人类结直肠腺癌细胞 (HT-29) 的增殖,并在体内促进了多的形成和组织学变化.
结论:
- 一种新型的Cronobacter malonaticus菌株 (PO3) 和其分泌的 (P506) 对结直肠癌细胞表现出增殖作用.
- 在小鼠模型中,P506表现出潜在的致癌活性,促进结直肠聚的发展和相关的组织学变化.
- 这些发现凸显了克罗诺细菌物种和特定分泌在人类结直肠癌进展中的潜在作用.
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