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精子sRNA的环境诱导变异与斑马鱼后代的基因表达和可移植元素有关
Alice M Godden1, Willian T A F Silva2,3, Berrit Kiehl2
1School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. Alice.Godden@uea.ac.uk.
Heredity
|March 23, 2025
概括
雄性斑马鱼暴露在不同的社会环境中,通过精子传递改变的小RNA (sRNA),影响后代的基因表达和可移植元素活性. 这揭示了父亲的环境压力如何影响后代的分子通路.
科学领域:
- 表观遗传学和跨代遗传学
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 环境因素可以通过父亲的影响影响后代的特征,可能由精子介导.
- 精子中的小RNA (sRNA) 参与基因组维护和对环境压力的反应,包括可移植元素 (TE) 活性.
- 了解男性社会环境如何影响sRNA和随后的后代基因表达是至关重要的.
研究的目的:
- 为了研究社会环境 (高与低竞争) 对雄性斑马鱼 (Danio rerio) 精子sRNA的影响.
- 确定父性sRNA,后代基因表达和可移植元素 (TE) 活性之间的联系.
- 开发用于将精子sRNA与胚胎基因表达和TE配置文件连接的计算工具.
主要方法:
- 两周内将雄性斑马鱼暴露在不同的社会环境中 (高/低竞争).
- 收集精子以分析差异表达的微RNA (miRNA) 和piwi相互作用RNA (piRNA).
- 使用暴露男性的精子进行体外受精,然后采集胚胎 (24hpf) 来评估基因和TE表达.
- 开发新的计算工具,以将精子sRNA与胚胎分子变化相关联.
主要成果:
- 在来自高竞争环境和低竞争环境的男性之间观察到精子sRNA概况的显著差异.
- 后代胚胎的基因表达和TE活动与父亲的社会环境有关.
- 计算分析确定了特定的精子sRNA与胚胎中改变的基因表达和TE活性之间的直接联系.
结论:
- 受到社会环境影响的男性生殖系中的分子应激反应对后代有显著的下游影响.
- 精子sRNAs充当调解者,将父亲的环境经验与下一代的基因表达和可移植元素调节联系起来.
- 这项研究提供了通过精子sRNA介导的表观遗传机制对社会环境跨代影响的直接证据.
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