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类细胞衍生的细胞外囊泡诱导巨细胞偏向M1类型的表型

V Acevedo-Sánchez¹, R M Rodríguez-Hernández¹, S R Aguilar-Ruíz¹

¹Facultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Ex Hacienda de Aguilera S/N, Calz. San Felipe del Agua, Oaxaca de Juárez, 68120, Oaxaca, Mexico.

Biochemical and biophysical research communications

|March 23, 2025

概括

来自正常和宫癌细胞的状细胞细胞外囊 (EV) 促进巨细胞向M1类炎症表型的两极分化,影响细胞因子分泌和反应性氧物种的产生.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

皮细胞衍生的细胞外囊泡 (EVs) 影响各种细胞类型,但它们在宫癌 (CC) 中的作用和对巨细胞的影响尚不清楚.
了解状细胞-EVs对免疫细胞的影响对于阐明CC病原性至关重要.

研究的目的:

为了研究瘤和非瘤角质细胞衍生的EVs对巨细胞两极分化的影响.
为了确定宫癌的EVs是否会影响巨细胞的行为.

主要方法:

使用外周血液单核细胞 (PBMC) 和THP-1细胞系来评估巨细胞两极化.
使用流式细胞计,ELISA和qRT-PCR分析了M1/M2标记物,细胞因子分泌 (IL-1β,IL-6,TGF-β,IL-10) 和基因表达.
还测量了反应性氧物种 (ROS) 的产生.

主要成果:

皮细胞衍生的EV显著增加了M1表型标记物 (CD80,CD86,HLA-DRα,TNF-α) 和促炎细胞因子 (IL-1β,IL-6).
来自非癌性角质细胞的EVs独特地增加了IL-10分泌.
来自宫癌角质细胞的EV显著增加了ROS的产生.

结论:

来自非瘤和CC来源的状细胞衍生的EVs在巨细胞中诱导M1类极化.
这些EVs调节巨细胞的炎症反应,可能有助于宫癌中的瘤微环境.

关键词:

宫癌是发生在宫癌的原因之一.细胞外囊泡中的细胞外囊泡.来自皮细胞的EVs.类似M1的表型巨细胞的两极分化

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