由介酶干细胞分泌的BDNF通过激活ERK1/2通路来改善老化的卵细胞质量和发育潜力
Jing Zhou1,2,3,4,5,6,7,8, Cheng Li1,2,3,4,5,6,7, Xin Mi1,2,3,4,5,6,7
1State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproductive Medicine, Institute of Women, Children and Reproductive Health, Shandong University, Jinan, Shandong, 250012, China.
Cell communication and signaling : CCS
|March 24, 2025
概括
中介细胞干细胞分泌物改善老年小鼠和人类的卵细胞质量. 大脑衍生神经营养因子 (BDNF) 是关键成分,通过ERK1/2通路激活来增强卵细胞的发育.
科学领域:
- 生殖生物学 生殖生物学
- 干细胞研究的研究.
- 老龄化的分子机制.
背景情况:
- 与年龄相关的卵细胞质量下降显著影响生育能力.
- 目前针对卵细胞质量下降的治疗方法有限.
- 中介细胞干细胞分泌体 (MSC-sec) 显示出改善卵细胞质量的潜力.
研究的目的:
- 在MSC-sec中确定负责改善卵细胞质量的活性成分.
- 阐明MSC-sec提高老年人卵细胞质量的分子机制.
- 研究MSC-sec及其组分在小鼠和人类卵细胞中的疗效.
主要方法:
- 免疫光和染色体扩散分析在老老鼠卵细胞上.
- 在体外和体内实验中使用BDNF及其中和抗体.
- 用RNA测序来分析母体mRNA的降解.
- 在体外培养老人卵子细胞.
主要成果:
- 通过MSC-sec治疗,改善了线组装,并减少了老鼠卵子细胞的形积分.
- 确定BDNF是MSC-sec中提高卵细胞质量的关键成分.
- BDNF激活了ERK1/2信号通路,增加了DAZL和BTG4的表达.
- 经过BDNF治疗,老年人卵子细胞的受精率和胚胎细胞形成率得到改善.
结论:
- 由MSCs分泌的BDNF有效地改善老鼠和人类的卵细胞质量和发育潜力.
- ERK1/2信号通路对于BDNF介导的卵细胞质量提升至关重要.
- BDNF是一个有前途的治疗策略,可以抵消与年龄相关的卵细胞质量下降.
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