网络药理学和实验验证揭示了Sishen Pill在治疗NSAID诱导的小肠的有效性
Jiaying Zhou1, Fengting Zhu1, Huixian Liang1
1Department of Gastroenterology, the Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, Yiwu, People's Republic of China.
西申药丸 (SSP) 通过减少炎症,氧化应激和调节PI3K/AKT通路来预防非类固醇抗炎药物诱导的小肠 (SIU). 这种传统中医药显示出作为一种有效的替代疗法的前景.
科学领域:
- 药理学 药理学是指药理学的学科.
- 胃肠病学 胃肠病学
- 传统中国医药 传统中国医药
背景情况:
- 非类固醇抗炎药物 (NSAIDs) 通常会导致小肠 (SIU),治疗选择有限.
- 传统中医药的西申药 (SSP) 显示出治疗SIU的潜力,但其机制需要阐明.
研究的目的:
- 系统地调查Sishen Pill (SSP) 对NSAID诱导的小肠的保护机制.
- 通过网络药理学,分子对接和体内验证,阐明SSP的多目标治疗效果.
主要方法:
- 网络药理学确定了SSP的活性成分及其标,并与SIU相关的基因进行了交叉引用.
- 进行了蛋白质-蛋白质相互作用网络和通路丰富分析 (GO,KEGG).
- 分子对接验证了配体-标相互作用,随后在鼠标模型上进行了因多美素诱导SIU的体内研究.
主要成果:
- SSP含有66种生物活性成分,针对222个基因,其中关键成分对AKT1和IL6等点具有很高的亲和力.
- 确定PI3K/AKT通路作为一个中心机制.
- 在体内,SSP显著降低了的严重程度,炎症和氧化应激,降低了PI3K和AKT1的表达.
结论:
- 通过多目标机制,SSP对NSAID诱导的SIU产生保护作用.
- SSP抑制炎症,氧化应激,并调节PI3K/AKT信号通路.
- 这些发现支持SSP作为SIU安全有效的替代疗法的潜力.
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