常见的抗微生物药物通过免疫心脏信号来破坏斑马鱼的早期发育
Yueyue Liu1,2, Chen Wang1, Zhiyou Fu1
1State key laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
Environmental science and ecotechnology
|March 24, 2025
概括
替代性抗微生物甲 (BAC) 对斑马鱼胚胎的发育风险高于受限制化学物质,如三三 (TCS). 这凸显了对新抗微生物药物的环境风险评估的需要.
科学领域:
- 环境毒理学环境毒理学
- 发展生物学 发展生物学
- 化学安全 化学安全
背景情况:
- 抗微生物化学品的使用在COVID-19后增加,引发了对水生动物发育毒性的担忧.
- 在环境度下,常见和替代抗微生物药物对发育的影响的数据有限.
研究的目的:
- 系统地比较三桑 (TCS),三卡班 (TCC),化 (BAC),化 (BEC) 和化 (CX) 在斑马鱼胚胎上的发育毒性.
- 调查抗微生物诱导的发育毒性背后的分子机制.
主要方法:
- 斑马鱼胚胎 (Danio rerio) 暴露于五种抗菌化学物质的不同度 (0.4,4,40μg L-1).
- 评估了发育终点 (化速率,心率,致性).
- 转录组分析,蛋白质-蛋白质相互作用网络和qPCR被用来识别受影响的分子通路.
主要成果:
- 化 (BAC) 导致化率和心率抑制的最严重降低.
- 所有测试化学品,除了氧烯 (CX) 引发显著的原性作用.
- 分子分析揭示了与免疫相关的凝血和MAPK信号通路的破坏,影响骨,心脏和免疫发育.
结论:
- 替代性抗微生物BAC在环境相关度下比限制性TCS和TCC带来更大的发育风险.
- 目前的风险评估可能低估了广泛使用的替代抗微生物药物的发育毒性,如BAC.
- 严格的环境风险评估对于替代抗微生物药物在广泛应用以保护水生生态系统之前至关重要.
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