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Updated: May 20, 2025

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells
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Published on: May 28, 2021

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在ToF-SIMS单细胞成像中,用于冷干燥样品制备的实验室友好协议

Xiujuan Shi1, Mingru Liu1, Yue Qi1

  • 1Key Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), State Ethnic Affairs Commission, Center for Imaging and Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing, China.

Frontiers in chemistry
|March 24, 2025
PubMed
概括

标准化一种新的冷干燥方法改善了用于飞行时间二次离子质谱 (ToF-SIMS) 的样本准备. 这提高了单细胞研究中的细胞分析和可重复性.

关键词:
在ToF-SIMS中使用.冰干燥的使用方法质谱仪成像成像 质谱仪成像成像样品的准备 样品的准备一个单细胞成像技术.

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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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