抑制铁亡减轻了绵羊精子结损伤
Erhan Hai1, Boyuan Li1, Yukun Song1
1Inner Mongolia Key Laboratory of Sheep and Goat Genetics Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Frontiers in veterinary science
|March 24, 2025
概括
低温保存通过亡和铁亡损害了绵羊的精子. 通过ferrostatin-1 (Fer-1) 抑制铁生变,显著改善了精子质量,并恢复了GPX4水平,为保存遗传物质提供了一个有前途的战略.
科学领域:
- 生殖生物学 生殖生物学
- 细胞死亡途径 细胞死亡途径
- 冰冷保存科学 冰冷保存科学
背景情况:
- 羊精子的冷保存可以诱导细胞损伤,涉及编程细胞死亡机制.
- 了解亡和铁亡的作用对于改善精子保存技术至关重要.
- 以前的研究表明,氧化应激会导致冷保存期间的精子受损.
研究的目的:
- 调查亡和铁亡在由冷保存诱导的绵羊精子损伤中的参与.
- 评估针对亡和铁亡途径的特定抑制剂在缓解冷保存损伤方面的有效性.
- 评估这些抑制剂对精子质量参数和关键分子标记物的影响.
主要方法:
- 在新鲜与冷保存的绵羊精子中比较apoptotic (Cleaved-caspase3) 和ferroptotic (TFRC) 标记物的表达.
- 应用的抑制剂:不同度的德菲洛克萨明甲基酸盐 (DFO),费洛斯塔丁-1 (Fer-1),利普洛克斯塔丁-1 (Lip-1) 和Z-VAD-FMK (Z-VAD).
- 评估了经解后的精子活力,血膜完整性,青体完整性,活性氧物种 (ROS),脂质过氧化,Fe2+水平和Glutathione Peroxidase 4 (GPX4) 表达.
主要成果:
- 冷保存增加了Cleaved-caspase3和TFRC的表达,表明并发的亡和铁亡.
- 最佳的抑制剂度 (DFO,Fer-1,Lip-1的2μM;Z-VAD的5μM) 显著改善了精子的运动性和膜完整性.
- 铁-1是最有效的,减少ROS,脂质过氧化和Fe2+,并恢复GPX4表达到新鲜的精子水平.
结论:
- 亡和铁亡都对羊精子的冷保存引起的损伤有显著的贡献.
- 铁素-1 (Fer-1) 有效地减轻铁亡,提高精子质量并恢复冷保存后的GPX4水平.
- 向铁死是改善精子冷保存结果的有希望的策略,有利于动物繁殖和保护工作.
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