通过PI3K-Akt路径,BmWARS可以抑制BmNPV感染
Jinyang Wang1,2, Xiangrui Ding1,2, Kaifang Jia1,2
1Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Bulletin of entomological research
|March 24, 2025
概括
博密克斯·莫里三甲基-tRNA合成酶 (BmWARS) 对博密克斯·莫里核多重体病毒 (BmNPV) 具有抗病毒特性. 过度表达BmWARS通过促进细胞亡和减少细胞增殖来抑制病毒复制.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 昆虫科学 昆虫科学 昆虫科学
背景情况:
- 三甲基-tRNA合成酶 (aaRSs) 对于蛋白质合成的忠实性至关重要.
- 博姆比克斯·莫里 (Bombyx mori Tryptophanyl-tRNA synthetase,简称BmWARS) 是一种在丝虫中发现的类似Ic的aaRS.
- 在此之前,BmWARS在抗病毒防御中的作用尚未被探索.
研究的目的:
- 从Bombyx mori.中克隆和描述BmWARS的特征.
- 研究BmWARS在丝虫对BmNPV感染的免疫反应中的作用.
- 阐明BmWARS抗病毒活性背后的分子机制.
主要方法:
- 克隆和BmWARS基因的表征.
- 在不同组织和病毒感染下分析BmWARS表达模式.
- 过度表达和干扰研究,以评估对BmNPV复制的影响.
- 研究PI3K-Akt通路中的关键基因表达.
主要成果:
- BmWARS被克隆,编码在细胞质中定位的382个氨基酸蛋白质.
- 在对BmNPV感染的反应中,BmWARS的表达上调,特别是在中肠和丝腺中.
- 过度表达BmWARS显著抑制了BmNPV复制,而干扰则促进了它.
- BmWARS调节PI3K-Akt通路,影响细胞亡和细胞增殖基因.
结论:
- 在Bombyx mori.中,BmWARS对BmNPV具有显著的抗病毒活性.
- BmWARS通过促进细胞亡和抑制细胞增殖来抑制病毒感染和复制.
- 这项研究揭示了BmWARS在昆虫对病毒病原体的天生的免疫力中的新角色.
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