相关实验视频
Updated: May 20, 2025
15:35
Analyzing Large Protein Complexes by Structural Mass Spectrometry
Published on: June 19, 2010
24.1K
高性能电荷检测质谱没有超高真空
Emeline Hanozin1, Conner C Harper1, Jacob S Jordan1
1Department of Chemistry, University of California, Berkeley, California, 94720-1460, USA. erw@berkeley.edu.
The Analyst
|March 24, 2025
概括
电荷检测质谱 (CDMS) 现在在更高的压力下工作,简化了测量. 这一进步允许在没有超高真空的情况下准确地确定大分子和纳米粒子的质量.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 物理化学 物理化学
背景情况:
- 电荷检测质谱 (CDMS) 传统上需要超高真空 (UHV) (<10-9 Torr).
- 超高频条件昂贵且繁,限制了CDMS的广泛应用.
- 与背景气体的碰撞可能会干扰CDMS中精确的离子测量.
研究的目的:
- 开发和验证一种CDMS方法,能够在比以前更高的压力下进行准确的测量.
- 为了研究高压对离子行为的影响和在静电陷中测量质量的准确性.
- 确定CDMS在非UHV条件下分析大型生物分子和纳米颗粒的可行性.
主要方法:
- 采用了一种电静电陷,旨在容纳广泛的离子能量.
- 实施了用于数据处理的动态离子信号分析方法.
- 在高达1×10-6的压力下对抗体复合体,病毒和聚烯纳米颗粒进行了CDMS测量 Torr.
主要成果:
- 在高达1×10−6Torr的压力下,实现了精确的CDMS质量测量,比以前的极限高出几个数量级.
- 成功测量了大型离子的质量,包括抗体复合体 (~800 kDa),腺相关病毒 (~4.8 MDa) 和纳米粒子 (35330 MDa).
- 证明较大的离子在更高的压力下表现出更大的强度,并且足够的较小离子存活下来以准确确定质量.
结论:
- 使用静电陷和动态分析,CDMS测量可以在高达1 × 10-6 Torr的压力下准确地执行.
- 这一进步大大减少了对昂贵和复杂的超高真空系统的需求.
- 这些发现为更容易获得的大分子和纳米粒子的常规质量分析铺平了道路.
相关概念视频
01:15High-Performance Liquid Chromatography: Types of Detectors
401
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
401
01:15High-Resolution Mass Spectrometry (HRMS)
1.2K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.2K
01:19Mass Analyzers: Common Types
536
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
536
01:21Mass Spectrometry: Complex Analysis
674
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
674
01:19Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
602
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
602
01:13Mass Analyzers: Overview
549
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
549