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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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相关实验视频

Updated: May 20, 2025

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
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在结直肠癌中解码非编码RNA机制的单细胞转录基因方法.

Mahnoor Naseer Gondal1,2, Hafiz Muhammad Umer Farooqi3

  • 1Department of Computational Medicine & Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA.

Non-coding RNA
|March 24, 2025
PubMed
概括

非编码RNAs (ncRNAs) 在结直肠癌 (CRC) 中至关重要. 本综述强调了单细胞生物信息学工具如何分析ncRNA表达和功能,从而推进CRC诊断和治疗策略.

关键词:
生物信息学是一种生物信息学.结肠直肠癌是什么意思没有编码的RNA.一个单细胞测序.治疗药物 治疗药物

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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相关实验视频

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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科学领域:

  • 基因组学和生物信息学
  • 癌症生物学 癌症生物学
  • 分子瘤学分子瘤学

背景情况:

  • 非编码RNAs (ncRNAs) 显著影响结肠直肠癌 (CRC) 的发展和进展.
  • 单细胞转录组分析揭示了CRC细胞的显著表达异质性,表明复杂的细胞格局.

研究的目的:

  • 在CRC内审查ncRNA研究的最新进展,特别关注单细胞生物信息学方法.
  • 探索使用CRC中的单细胞RNA测序 (scRNA-seq) 数据进行ncRNA识别,表征和功能预测的计算方法.

主要方法:

  • 对计算策略的审查,包括基于序列,基于结构,机器学习和多omics集成用于ncRNA分析.
  • 生物信息技术的应用用于差异表达分析,功能丰富和CRCncRNAs中的调控网络构建.
  • 通过scRNA-seq数据分析检查CRC中的ncRNA异质性.

主要成果:

  • 生物信息学工具对于识别,描述和预测CRC中ncRNAs的功能至关重要.
  • 计算方法可以分析差异性ncRNA表达,功能丰富和监管网络.
  • scRNA-seq数据分析揭示了CRC内的显著ncRNA异质性,为瘤生物学提供了新的见解.

结论:

  • 单细胞生物信息学对于理解CRC中的ncRNA生物学至关重要.
  • 计算方法与实验验证的整合是通过ncRNA研究推进CRC诊断,预后和治疗策略的关键.