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Updated: May 23, 2025

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基于瞳孔的近距离标记揭示了阿拉比多普西斯TOR复合体的综合蛋白质和蛋白相互作用体

Shuai Zheng¹, Leonard Blaschek¹, Delphine Pottier¹

¹Copenhagen Plant Science Center (CPSC), Department of Plant & Environmental Sciences, University of Copenhagen, Frederiksberg C, 1871, Denmark.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|March 24, 2025

概括

研究人员使用近距离标记在植物细胞中绘制了拉巴胺复合体 (TORC) 标的蛋白质相互作用. 这项研究揭示了超过一百个新的相互作用体,推进了植物细胞信号知识.

科学领域:

植物细胞生物学植物细胞生物学
分子信号通道的分子信号通道.
蛋白质组学是指蛋白质组学.

背景情况:

拉巴胺素的目标 (TOR) 信号通路对于整合各种信号来调节植物生长和碳分配至关重要.
核心TOR复合体 (TORC) 由TOR,LST8-1和RAPTOR1组成,但其完整的蛋白质-蛋白质相互作用网络 (相互作用体) 仍然在很大程度上没有表征.

研究的目的:

为了全面地绘制植物细胞中的TORC互动组.
为了确定新型蛋白相互作用和TOR激酶的潜在直接标.
调查TORC相互作用如何受到碳可用性的影响.

主要方法:

适应和应用PUP-IT (蛋白质非近距离诱导标签) 工具箱用于内源蛋白质近距离标签.
使用核心TORC组件作为诱蛋白来捕获相互作用的合作伙伴.
使用AlphaFold-Multimer进行识别互动器的计算验证.

主要成果:

创建了一个全面的TORC交互体,揭示了超过一百个新的候选交互体.
在不同碳可用性条件下观察到相互作用体内的不同酸化模式.
确定了TOR激酶活性的假定直接点.

结论:

这项研究为了解植物细胞信号和TORC功能提供了宝贵的资源.
鉴定到的交互原子和酸化数据为植物如何管理碳分配和生长提供了新的见解.
这项工作为未来研究植物中TORC介导的调节机制奠定了基础.

关键词:

阿尔法折叠是什么意思阿尔法折叠在PUP-IT中,PUP-IT是PUP-IT.接近性标签的标签.糖的信号表示糖.拉帕米的目标是拉帕米.

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