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在Saccharomyces pastorianus细胞中氧化损伤和DNA突变,这些细胞被压缩二氧化碳微气泡处理

Fumiyuki Kobayashi^1,2, Jun-Ichi Shiraishi³, Konomi Takahashi^3,4

¹Faculty of Applied Life Science, Nippon Veterinary and Life Science University, Musashino, Tokyo, 180-8602, Japan. fkoba@nvlu.ac.jp.

Current microbiology

|March 24, 2025

概括

压力下的二氧化碳微气泡 (CO2MB) 在Saccharomyces pastorianus细胞中引起DNA损伤和基因突变. 这些影响,包括氧化应激和DNA碎片化,随着更高的温度和更长的暴露时间而加剧.

科学领域:

生物技术是生物技术.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

压缩二氧化碳微气泡 (CO2MB) 越来越多地被用于各种生物应用.
了解它们对像DNA这样的细胞组件的影响对于安全性和有效性评估至关重要.
萨卡罗米切斯 (Saccharomyces pastorianus) 作为一种模型生物,用于研究细胞对物理和化学处理的反应.

研究的目的:

为了研究CO2MB对Saccharomyces pastorianus细胞DNA的影响.
为了确定温度和暴露时间如何影响CO2MB诱导的DNA变化.
评估CO2MB处理引起的DNA损伤类型和遗传变异.

主要方法:

用醇提取和阿加罗斯凝电泳来分析DNA度和完整性.
使用定量PCR测量DNA碎片化.
对apurinic/apyrimidinic位点的分析表明氧化DNA损伤.
基于单核酸多态性和插入/删除分析的遗传学分析揭示了遗传变异.

主要成果:

DNA度显示温度变化的变量,而凝上的DNA带密度在更高的温度 (40-45°C) 上增加.
随着温度和暴露时间的增加,DNA碎片化减少,但氧化DNA损伤 (阿普里尼克/阿普里米尼克位点) 增加.
CO2MB处理导致了更大的遗传分歧,增加了单核酸多态,并在S. pastorianusDNA中增加了插入/删除,特别是在高温和更长时间的情况下.

结论:

在Saccharomyces pastorianus中,CO2MB会诱导部分氧化DNA损伤和突变.
观察到的DNA变化很可能归因于细胞内产生的氧化应激.
温度和暴露时间是调节CO2MB诱导的DNA损伤和遗传变异程度的关键因素.

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