相关实验视频
Updated: May 20, 2025
10:06
Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
7.6K
在人类和老鼠肝脏中对功能性保存的长非编码RNA代谢调节物的系统识别
Chengfei Jiang1, Zhe Li1, Sunmi Seok1
1Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland.
Gastroenterology
|March 24, 2025
概括
确定了功能性保存的长非编码RNA代谢调节剂 (fcLMRs),证明了人类和小鼠非编码RNA之间共享的肝功能. 一种特定的fcLMR,h/mLMR1,通过与PABPC1相互作用来调节甘油三水平,为肝硬化提供了潜在的治疗标.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 大多数人类长非编码RNA (lncRNAs) 缺乏序列保存,使功能研究和临床翻译复杂化.
- 这项研究调查了这样的假设,即非保存的lncRNAs可以在物种中共享保存的代谢功能,称为功能保存的lncRNA代谢调节器 (fcLMRs).
研究的目的:
- 为了识别和描述人类和小鼠肝脏之间的fcLMRs.
- 阐明fcLMRs调节基因表达和代谢过程的分子机制.
- 探索非保存的lncRNAs在肝脏疾病 (如肝硬化症) 中的治疗潜力.
主要方法:
- 开发了一种独立于序列的策略来识别假定的fcLMRs.
- 对人类和小鼠的lncRNA对 (h/mLMRs) 进行了比较功能分析.
- 研究了一种特定的fcLMR (h/mLMR1) 的作用机制,涉及PABPC1相互作用和下游信号通路.
主要成果:
- 确定了几种表现出类似基因调节功能的假定fcLMRs.
- 证明h/mLMR1在两种物种中都能调节甘油三水平和脂原基因表达.
- 阐明了h/mLMR1通过结构相似的动机与PABPC1结合,抑制翻译并激活氨基酸-mTOR-SREBP1c通路.
- 表明PABPC1结合基因完全挽救了跨物种的lncRNA功能.
- 在肝硬化模型中观察到升高的h/mLMR1表达.
结论:
- 功能性保存的lncRNA代谢调节器 (fcLMRs) 是一种新奇且广泛的生物现象.
- 对于研究人类肝脏病理生理学而言,lncRNA功能的小鼠模型非常有价值.
- hLMR1的PABPC1结合基因是非保存的lncRNA介导疾病的潜在药物标,如肝硬化症.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.4K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K
02:51Multi-species Conserved Sequences
3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K
01:56Riboswitches
8.0K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.0K
02:24Ribosome Profiling
3.4K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.4K