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在Prunus serrula Franch基因组的染色体层次组合

Hao Zuo^1,2,3,4, Shengjun Liu^2,3, Lei Tan^2,3

¹Qinghai-Tibet Plateau Fruit Trees Scientific Observation Test Station (Ministry of Agriculture and Rural Affairs), Lhasa, Tibet, 850032, China.

Scientific data

|March 25, 2025

概括

我们为P. serrula提供了高质量的基因组组,P. serrula是一种适应高海拔地区的植物物种. 这种遗传资源有助于理解高海拔适应机制.

科学领域:

基因组学就是基因组学.
植物生物学植物生物学
进化生物学进化生物学

背景情况:

*P. serrula是一种在中国高海拔地区发现的植物物种.
*了解其遗传构成对于研究适应极端环境至关重要.

研究的目的:

*为P. serrula.生成染色体级的基因组组合.
* 提供遗传资源用于遗传学研究.
* 为了研究高海拔适应的遗传基础.

主要方法:

* 利用了Illumina测序,牛津纳米孔超长测序和Hi-C技术.
* 采用生物信息学组装和注释基因组.
*使用BUSCO评估基因组的完整性.

主要成果:

*一个284.5 Mb的基因组组合,一个32.4 Mb的N50支架.
*91.9%的组件在8个伪染色体上.
*35151个蛋白质编码基因和47340个转录被注释,BUSCO完整度为98.5%.

结论:

*高质量的基因组组合为P. serrula提供了宝贵的遗传资源.
* 提供了对基因架构的洞察,使得高海拔适应.
* 促进植物基因组学和进化研究的未来研究.

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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.