储存纤维,纤维束和整个肌肉在糖溶液中的效果对它们的被动弹性模量
Iraj Dehghan-Hamani1,2, Stephen H M Brown3, Thomas R Oxland4,5,6
1Department of Mechanical Engineering, University of British Columbia, Vancouver, BC, Canada.
Scientific reports
|March 25, 2025
概括
甘油溶液显著降低了大约50%的肌肉纤维和肌肉束的弹性模量. 在这种溶液中储存肌肉组织会影响被动机械性能,并可能导致损伤,特别是在整个肌肉中.
科学领域:
- 生物机械工程 生物机械工程
- 肌肉生理学 肌肉生理学
- 材料科学 材料科学 材料科学
背景情况:
- 在糖溶液中保存骨肌肉是生物力学测试的常见方法.
- 了解保存对机械性能的影响对于准确的研究至关重要.
研究的目的:
- 调查糖溶液储存对骨肌肉的被动弹性模量在纤维,纤维束和整个肌肉尺度上的影响.
- 为了比较新鲜肌肉样本的机械性能与存储在糖溶液中的机械性能.
主要方法:
- 拉伸测试是在斯普拉格-道利大鼠的前肌肉上进行的.
- 样本 (纤维,纤维捆,整个肌肉) 进行新鲜测试或在-20°C的糖溶液中储存2周.
- 弹性模块是从10%,20%和30%应变的应力-应变曲线计算出来的.
主要成果:
- 在纤维和捆束中,糖溶液的储存使弹性模量降低了50% (p<0.001).
- 整个肌肉储存导致中央纤维损伤,质量减少和生理横截面积减少 (p<0.002).
- 储存的纤维/捆绑显示松散肉瘤的长度减少,横截面积增加 (p<0.041).
结论:
- 糖溶液的储存显著改变肌肉组织的被动机械特性,特别是在纤维和捆绑层面.
- 储存效应因组织规模而异,整个肌肉经历内部损伤.
- 储存的纤维/束的拉伸测试主要评估被动性质,而新鲜的标本提供了对被动性质和活性性质的洞察.
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