由Bacillus megaterium诱导的酸导致Capsicum chinense中对领的系统性抵抗
Priyanka Gogoi1,2, Bhaben Sharmah3,2, Prasenjit Manna3,2
1Biological Sciences and Technology Division (BSTD), CSIR-North East Institute of Science and Technology, Jorhat, 785006, Assam, India.
Plant cell reports
|March 25, 2025
概括
巨杆菌JPR68通过增加酸 (SA) 水平来增强Bhut Jolokia植物的免疫力. 这增强了对Rhizoctonia solani的抵抗力,减少了子病和植物损伤.
科学领域:
- 植物病理学 植物病理学
- 微生物学 微生物学
- 植物生理学 植物生理学
背景情况:
- 酸 (SA) 是一种植物激素,对植物免疫反应至关重要,包括系统性获得性抵抗 (SAR) 和诱导性系统性抵抗 (ISR).
- 作为ISR中针对Rhizoctonia solani等真菌病原体的信号分子,SA的确切作用仍然是积极研究的领域.
- 布特乔洛基亚 (Capsicum chinense Jacq.) 是一种植物. 容易患上领病,影响作物产量和质量.
研究的目的:
- 调查Bacillus megaterium JPR68在诱导Capsicum chinense Jacq.的系统性耐药性的作用.
- 为了确定酸 (SA) 在植物对抗Rhizoctonia solani的防御机制中的参与.
- 评估SA在管理Bhut Jolokia的领病的有效性.
主要方法:
- 在体外抑制Rhizoctonia solani菌体生长,使用不同度的酸 (SA).
- 在Capsicum chinense Jacq.上进行致病性测定. 用SA处理的叶子和水果,以评估R. solani生长抑制.
- 分裂根技术分析SA积累在根组织中接种后与Bacillus megaterium JPR68 (BmJPR68).
- 测量反应性氧物种 (ROS) 生产和检测受治疗的植物中与病变发生相关的蛋白质 (PR).
主要成果:
- 巨杆菌JPR68显著增强诱导的系统性耐药性 (ISR) 在红豆 Jacq.,导致SA水平升高.
- 在实验室中,SA证明了Rhizoctonia solani菌体生长的强烈抑制,在15mM时完全停止了它的生长.
- 在植物中,SA治疗显著降低了项圈腐烂症状,亡和化,同时阻碍了R. solani在叶子和果实上的生长.
- 在细菌化根组织中观察到SA积累,BmJPR68治疗减少了活性氧物种 (ROS) 生产.
- 里埃变换红外线 (FT-IR) 分析证实了与植物防御反应相关的功能组,并检测到与病变发生相关的 (PR) 蛋白质.
结论:
- 巨杆菌JPR68有效地诱导了Capsicum chinense Jacq. 的系统性耐药性. 通过酸 (SA) 生产.
- 酸在缓解Rhizoctonia solani感染和Bhut Jolokia的领病方面发挥着至关重要的作用.
- 这项研究强调了使用BmJPR68通过SA诱导对中的真菌病进行生物控制的潜力.
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