在生物和非生物压力下,Saccharum复合体中CAD类家族基因的功能分歧
Zhu-Qing Wang1,2, Sheng-Ren Sun1,2, Hao-Yu Wang3
1Institute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou, 510000, China.
Plant cell reports
|March 25, 2025
概括
研究人员在甘中确定了54个膜攻击复合物/福林 (MACPF) 超级家族基因,揭示了植物对压力因素的防御中的多种功能. 这些发现为开发抗病甘作物提供了新的资源.
科学领域:
- 遗传学和分子生物学
- 植物科学 植物科学
- 免疫学 免疫学 免疫学
背景情况:
- 膜攻击复合物/福林 (MACPF) 超级家族包括形成毛孔的蛋白质,这些蛋白质对于真核生物的先天性和适应性免疫至关重要.
- 含有MACPF域的构成性激活细胞死亡 (CAD) 蛋白质与植物对环境压力因素的防御机制有关.
- 在甘中CAD类基因的特定作用和特征仍然在很大程度上未被探索.
研究的目的:
- 在Saccharum复合体内识别和表征CAD类基因.
- 研究这些基因在应对各种压力时的表达模式和功能作用.
- 为增强甘抗病能力提供潜在的基因资源.
主要方法:
- 来自Saccharum复合体 (Saccharum spontaneum和Erianthus rufipilus) 的54个CAD类基因的全基因识别.
- 遗传学分析将基因分类为五组.
- 在促进区域中分析cis-acting元素.
- 转录组和RT-qPCR分析以评估在压力条件下的基因表达 (寒冷,干旱,ABA,SA,Xanthomonas albilineans).
- 使用转基因Arabidopsis线的功能分析.
主要成果:
- 确定了54个类似CAD的基因,并将其分为五个基因组.
- 促进器区域含有与压力和植物激素反应相关的 cis 作用元素.
- 基因在各种非生物和生物压力治疗下表现出多样化的表达特征.
- 在冷,干旱,ABA和SA治疗下,以及在Xanthomonas albilineans感染时,SsaCAD1-like1显示出差异调节.
- 在非生物压力下,SsaCAD4-like1和SsaCAD2-like等位基因通常被下调.
- 在Arabidopsis中,SsaCAD1-like1发挥了负面作用,而SsaCAD3-like2/4对抗细菌感染发挥了积极作用.
结论:
- 该研究在Saccharum复合体中发现了大量的MACPF超级家族基因.
- 在SsaCAD类基因中存在功能差异,这表明植物在抵抗压力因素的防御中扮演着不同的角色.
- 这些已识别的基因代表了繁殖抗病甘品种的宝贵资源.
更多相关视频
08:11Author Spotlight: Understanding Microbe Adaptation Using Innovative Techniques for Exploring Thermophilic Evolution
Published on: June 14, 2024
651
06:04Author Spotlight: Innovative Approaches to Understanding Plant Structure-Function Relationships for Climate-Resilient Crops
Published on: July 12, 2024
816
相关概念视频
C4 Pathway and CAM
45.1K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.1K
Adaptations that Reduce Water Loss
25.0K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
25.0K
The Calvin Benson Cycle
4.4K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.4K
Responses to Salt Stress
12.9K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
12.9K
Frequency-dependent Selection
21.7K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
21.7K
Responses to Drought and Flooding
10.6K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
10.6K
