作为太平洋白 (Litopenaeus vannamei) 的益生菌,土著的阴性细菌
Sutanti Sutanti1,2, Sukenda Sukenda3, Widanarni Widanarni1
1Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Campus IPB, Dramaga Bogor, West Java, 16680, Indonesia.
Current microbiology
|March 25, 2025
概括
本土格兰氏阴性细菌益生菌显著改善了太平洋白的生长,免疫力和消化酶. 这种可持续的方法减少了水产养殖中的抗生素使用.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 微生物学 微生物学
- 动物科学动物科学
背景情况:
- 益生菌增强水产养殖中的生长,免疫力和繁殖能力.
- 益生菌为抗生素提供了一种安全的替代品,用于对抗耐药细菌.
研究的目的:
- 为了评估本地格兰氏阴性细菌 (Shewanella藻类A3和Serratia marcescens Van80 UB3) 作为太平洋白 (Litopenaeus vannamei) 的益生菌.
- 评估新型益生菌菌株的安全性和抗生素耐药性.
- 确定单种和多种益生菌饮食对生长,免疫力和基因表达的影响.
主要方法:
- 一个为期30天的食试验,使用幼后的 (PL-10),补充了10^6 cfu/g的饮食.
- 抗生素耐药性评估的表型和分子方法.
- 分析生长性能,消化酶活动 (脂酶,氨酶,蛋白酶),组织结构和与生长相关的基因表达 (IGF-1,ecdysteroid,myosin).
主要成果:
- 多原生益生菌组显示显著更高的脂酶,粉酶和蛋白酶活动 (P < 0.01).
- 与单个益生菌组相比,多益生菌组的生长性能优越 (P < 0.05).
- 多益生菌组中与生长相关的基因表达显著增加 (P < 0.05).
结论:
- 用10^6 cfu/g的多原生格兰氏阴性细菌益生菌的食补充剂可以增强太平洋白的生长,免疫力和消化酶活性.
- 这些益生菌代表了养殖的可持续实践,减少了对抗生素的依赖.
- 测试的菌株在水产养殖应用中显示出良好的安全性.
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