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鉴定CCNB1作为肝细胞癌中细胞衰老的生物标志物:生物信息学和实验验证研究

Zhilan Zhang¹, Jie Zhou¹, Ruiru Huang¹

¹College of Pharmacy, Anhui Medical University, Hefei, 230000, Anhui, China.

Discover oncology

|March 25, 2025

概括

这项研究确定CCNB1是肝细胞癌 (HCC) 进展和衰老的关键因素. CCNB1显示出作为诊断生物标志物和HCC的潜在治疗点的前景.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

肝细胞癌 (HCC) 缺乏有效的诊断生物标志物和治疗点.
早期的HCC通常无症状,导致晚期诊断和不良预后.
细胞衰老在HCC的发展和进展中起着重要作用.

研究的目的:

为了研究细胞衰老对HCC的影响.
为了确定HCC.潜在的诊断生物标志物和治疗点.
探索与衰老相关的差异表达基因 (AgHDEGs) 在HCC.中的作用.

主要方法:

集成的多个基因表达总体 (GEO) 微阵列数据集.
用R包的limma进行差异性基因表达分析.
雇佣权重基因共同表达网络分析 (WGCNA) 以确定关键基因模块和枢纽基因.
进行了基因本体学 (GO) 和KEGG通路丰富分析.
通过体外实验和分子对接模拟验证的结果.

主要成果:

确定了八个关键的AgHDEG,其中CCNB1被突出显示为一个重要的枢纽基因.
通过ROC曲线分析,CCNB1显示出强大的诊断潜力.
在HCC中,CCNB1表达是上调调的,与M0巨细胞相关.
抑制CCNB1降低了HepG2细胞的增殖,并促进了衰老.
分子对接建议CCNB1作为潜在的治疗点.

结论:

CCNB1与HCC衰老和进展有关.
CCNB1作为HCC的诊断生物标志物具有前途.
CCNB1代表了HCC治疗的潜在治疗标.

关键词:

生物信息学分析生物标志物生物标志物在CCNB1上,我们可以看到.细胞衰老细胞衰老实验验证实验验证的验证肝细胞癌是肝细胞癌.

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