对临床体液样本中致病原体鉴定的下一代元基因组测序进行比较分析
Ning Sun1, Jiaxun Zhang2, Wentao Guo3
1Department of Clinical Laboratory, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China. steamsn@163.com.
BMC microbiology
|March 25, 2025
概括
全细胞DNA元基因组下一代测序 (mNGS) 在体液样本中对病原体检测的灵敏度高于无细胞DNA mNGS和16S rRNA NGS. 由于特异性较低,需要仔细解释.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 临床诊断 临床诊断 临床诊断
背景情况:
- 甲基因组下一代测序 (mNGS) 为病原体识别提供了独立于培养的方法.
- 评估不同的mNGS方法,如全细胞DNA (wcDNA) 和无细胞DNA (cfDNA),对于优化临床诊断至关重要.
研究的目的:
- 为了比较wcDNA mNGS,cfDNA mNGS和16S rRNA测序在临床体液样本中检测病原体的有效性.
- 为了评估wcDNA mNGS的诊断性能与培养结果相比.
主要方法:
- 使用mNGS向wcDNA的125个临床体液样本的分析.
- 使用cfDNA mNGS对30个样本和使用16S rRNA NGS对41个样本进行比较分析.
- 测序结果与患者临床数据和培养结果的相关性.
主要成果:
- 与cfDNA mNGS (95%) 相比,wcDNA mNGS的宿主DNA比例 (84%) 显著较低.
- 对于wcDNA mNGS (63.33%) 与cfDNA mNGS (46.67%) 的结果,与培养结果的一致性更高.
- 与16S rRNA NGS (58.54%) 相比,wcDNA mNGS表现出更高的细菌检测一致性 (70.7%),并实现了74.07%的灵敏度和56.34%的特异性.
结论:
- 与cfDNA mNGS和16S rRNA NGS相比,wcDNA mNGS在体液中对病原体识别具有更高的灵敏度,特别是对于腹部感染.
- wcDNA mNGS的较低特异性需要仔细的临床解释,以避免假阳性.
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