使用生物分析,多重CRISPR/Cas9编辑和独立选择性PCR进行玉米杂交的高效突变和基因定型

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Maruti Nandan Rai1,2, Brian Rhodes1, Stephen Jinga1

  • 1Department of Crop Sciences, College of Agricultural, Consumer and Environmental Sciences (ACES), University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA.

Plant methods
|March 25, 2025
PubMed
概括

这项研究引入了玉米的简化CRISPR/Cas9基因组编辑系统,使多重基因编辑和高效的基因型定型成为可能. 这种方法加速了跨多种玉米品种的功能基因组学研究.

关键词:
基因编辑 基因编辑通过高吞吐量基因型鉴定.部分选择性PCR (IS-PCR) 进行.

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