循环RNA circDCUN1D4通过准miR-590-5p/TIMP3轴抑制肝细胞癌的发展
Hongyu Li1,2, Bing Su3, Yan Jiang4
1College of Chemistry and Lie Science, Beijing University of Technology, Beijing, 100124, China. leinade123@126.com.
Molecular cancer
|March 25, 2025
概括
循环RNA circDCUN1D4显示出治疗肝细胞癌 (HCC) 的前景. 更高的circDCUN1D4水平与更好的生存率和抑制瘤生长相关,为肝癌提供了潜在的新疗法.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 肝细胞癌 (HCC) 是一个重大的全球健康挑战.
- 对于有效的HCC治疗,迫切需要新的治疗策略.
- 循环RNAs (circRNAs) 正在成为各种癌症的关键调节者.
研究的目的:
- 为了研究circRNAcircDCUN1D4在HCC进展中的功能作用.
- 探索circDCUN1D4在HCC中的治疗潜力.
- 阐明 circDCUN1D4 在 HCC.中所起作用的分子机制.
主要方法:
- 在HCC患者中分析circDCUN1D4表达水平.
- 在体外测试以评估circDCUN1D4对HCC细胞增殖,迁移和入侵的影响.
- 确定circDCUN1D4的分子标的机制研究,包括miRNA海绵化.
- 使用小鼠模型进行体内研究,以评估circDCUN1D4的治疗疗效和毒性.
主要成果:
- 在HCC患者中,更高的circDCUN1D4表达与改善的生存率有关.
- circDCUN1D4显著抑制了HCC细胞的增殖,迁移和入侵.
- circDCUN1D4作为miR-590-5p的海绵,降低了其目标基因,组织金属蛋白酶3抑制剂 (TIMP3) 的下调.
- 在体内给予circDCUN1D4有效抑制了小鼠的瘤进展,没有显著的毒性.
结论:
- circDCUN1D4在抑制HCC进展方面发挥着至关重要的作用.
- miR-590-5p/TIMP3轴是通过circDCUN1D4发挥抗瘤作用的关键机制.
- circDCUN1D4代表了对HCC的有希望的治疗候选者,具有基于circRNA的治疗潜力.
关键词:
肝细胞癌是肝细胞癌.时间表3 TIMP3圆圈DCUN1D4D1D1D4D1D4D1D4D1D4D1D4D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D1D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2D2在 miR-590-5p 中使用.
相关概念视频
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:39lncRNA - Long Non-coding RNAs
8.4K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K
02:15Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
02:23Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
02:34Inhibition of Cdk Activity
4.6K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.6K
01:12Loss of Tumor Suppressor Gene Functions
4.7K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.7K