在BCG治疗期间NMIBC患者的尿路微生物群变化:比较BCG响应者和不响应者
Toni Boban1, Blanka Milić Roje2, Dora Knezović2
1Department of Urology, University Hospital of Split, Split, Croatia.
Frontiers in cellular and infection microbiology
|March 25, 2025
概括
尿液微生物群的组成可以预测Bacillus Calmette-Guérin (BCG) 免疫疗法在膀癌中的有效性. 在非响应者中观察到特定的细菌变化,这表明尿路微生物群在治疗结果中起着作用.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 微生物学 微生物学
- 在瘤学瘤学.
背景情况:
- 高风险的非肌肉侵入性膀癌 (NMIBC) 通常用通过尿道切除和Bacillus Calmette-Guérin (BCG) 免疫疗法治疗.
- 由于缺乏可靠的生物标志物,预测BCG治疗反应仍然是一个临床挑战.
- 新兴研究表明,尿路微生物群对BCG疗效的潜在影响.
研究的目的:
- 为了研究BCG免疫治疗周期期间尿路微生物群组成的变化.
- 探索尿路微生物群对NMIBC患者BCG治疗结果的预测能力.
主要方法:
- 使用从导管样本中16SrRNA基因测序对尿道微生物群的分析.
- 采集的样本在治疗前和在每六个BCG诱导剂量之前收集.
- 纳入标准:男性,没有先前的泌尿器癌症,没有其他恶性瘤,没有活跃的感染,最近没有使用抗生素.
主要成果:
- 大多数患者在BCG治疗的第一周观察到尿路微生物多样性的显著减少 (香农指数).
- 不同的尿路微生物群概况在治疗前区分了BCG响应者与不响应者.
- 非响应者显示出明显更高的Aureispira (12倍,p<0.001) 和较低的Negativicoccus succinivorans (27倍,p<0.001) 的丰度.
结论:
- 尿路微生物群组成在BCG治疗期间表现出动态变化,可以作为治疗反应的预测生物标志物.
- 特定的细菌系,如Aureispira,可能与对BCG免疫疗法的不反应有关.
- 需要在更大的患者队伍中进行进一步的验证,以确认尿道微生物群在NMIBC管理中的作用.
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