在有或没有抗逆转录病毒治疗的致病性SIV感染期间的血脂组变化
Sindhuja Sivanandham1,2, Ranjit Sivanandham1,2, Cuiling Xu1,2
1Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, United States.
类似人体免疫缺陷病毒 (SIV/HIV) 感染会改变脂质特征,影响炎症和并发性疾病. 控制的动物模型显示了感染和抗逆转录病毒治疗 (ART) 期间的特定脂质变化,为艾滋病毒相关疾病提供了潜在的生物标志物.
科学领域:
- 利皮多米克 (Lipidomics) 是一种消化剂.
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
背景情况:
- 在人类免疫缺陷病毒 (HIV) 感染时,脂质样本发生变化,与炎症相关.
- 行为风险因素使理解与艾滋病毒相关的脂质变化变得复杂,需要控制的动物模型.
研究的目的:
- 在受控动物模型中调查由猿类免疫缺陷病毒 (SIV) 感染和抗逆转录病毒疗法 (ART) 诱导的特定脂质组变化.
- 为了将脂质变化与关键感染参数相关联,并确定HIV/SIV并发症的潜在生物标志物.
主要方法:
- 超高性能液态染色学-并联质谱学 (UHPLC-MS/MS) 用于分析感染SIV的猪尾 (PTMs) 的纵向脂质变化.
- 使用ANOVA比较原始和ART治疗的SIV感染PTM之间的脂质度.
- 关键的感染参数 (IL-6,TNFa,D-二次体,CRP,CD4+T细胞计数) 与使用斯皮尔曼相关性与脂质水平相关.
主要成果:
- 在急性SIV感染期间,斯芬戈米林和乳糖胺增加,而糖胺在整个感染期间增加,并在ART时正常化.
- 酸氨基和溶酸胆在SIV感染下降,并没有在ART治疗中恢复.
- 酸乙烯胺,酸乙烯胺和酸胆因因SIV而没有变化,但随着ART而减少. 特定的脂类物种被SIV和/或ART显著改变,与疾病进展和炎症相关.
结论:
- SIV感染和ART诱导不同的脂质组签名,其中一些反映了艾滋病毒感染者 (PWH) 的情况.
- 鉴定的脂质变化与器官功能障碍标志物有关,并可能作为SIV/HIV并发症的生物标志物.
- 向失脂症可能对预防与艾滋病毒相关的并发症至关重要.
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