多功能益生菌通过层层地涂层酸-Mg2+和素合物复合物来预防性结肠炎
Zhanyin Qian1, Zihan Zhai2, Mingjin Ren1
1The Province and Ministry Co-Sponsored Collaborative Innovation Center for Medical Epigenetics, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China.
一种新的涂层策略增强了益生菌的传递和功能,通过调节肠道微生物群和减少炎症,为预防性结肠炎 (UC) 提供了一种有希望的方法.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 生物材料科学 生物材料科学
背景情况:
- 性结肠炎 (UC) 是由肠道微生物群失衡,炎症和氧化应激驱动的.
- 益生菌为UC预防提供了抗生素的潜在替代品,但在胃肠道中面临着传递挑战.
- 开发强大的益生菌输送系统对于有效的UC管理至关重要.
研究的目的:
- 开发一种有效的益生菌表面涂层策略,以提高其稳定性和治疗效果.
- 创建能够抵抗胃肠道疾病并提供治疗效益的多功能益生菌.
- 在小鼠模型中评估涂层益生菌在预防硫酸 (DSS) 诱导的大肠炎方面的疗效.
主要方法:
- 用酸 (TA) -Mg2+和酸 (CPP) 复合体对*Saccharomyces boulardii* (SB) 进行层层的涂层.
- 在模拟的胃和肠液中评估涂层益生菌 (SB@TA-Mg2+@CPP) 的稳定性.
- 在DSS诱导的大肠炎小鼠模型中评估SB@TA-Mg的抗氧化活性,细胞活力和对肠道微生物群的影响.
主要成果:
- SB@TA-Mg2+@CPP表现出显著增强的稳定性,细胞活力和抗氧化活性.
- 涂层益生菌在DSS诱导的大肠炎模型中表现出强大的抗炎和抗氧化作用.
- SB@TA-Mg2+@CPP治疗增加了肠道微生物群的丰富性和多样性,改善了肠道屏障的完整性,并增强了Mg2+吸附.
结论:
- 开发的TA-Mg2+-CPP涂层策略有效地保护益生菌,并赋予多种治疗功能.
- 多功能益生菌通过解决关键的病理机制,显示出预防性结肠炎的巨大潜力.
- 这种方法为开发用于炎症性肠道疾病的先进益生菌疗法提供了有希望的途径.
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