用超高性能液体色谱法确定胰岛素,并联质谱法增强了基于Mg的微电机
Jing Li1, Qingtao Song2, Yuliang Chen2
1College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Mikrochimica acta
|March 25, 2025
概括
这项研究引入了基于Mg的微电机,用于增强药物输送和检测. 这些活性微驱动器改善了肠道药物保留,并使用超高性能液态染色体质谱法 (UPLC-MS) 实现了对胰岛素的敏感检测.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术纳米技术
- 分析化学 分析化学
背景情况:
- 克服粘液屏障对于有效的肠道药物输送至关重要.
- 目前的药物检测方法在复杂的生物矩阵中扎着低度的药物.
- 需要积极的药物输送系统来提高治疗疗效和诊断灵敏度.
研究的目的:
- 开发一种使用基于Mg的微电机的活性药物递送载体,用于增强肠道药物检测.
- 调查基于Mg的微电机克服粘液屏障和改善药物保留的能力.
- 使用超高性能液态染色体质谱法 (UPLC-MS) 进行微电机传递的胰岛素的敏感检测.
主要方法:
- 基于Mg的微电机 (Mg/Au/PLGA/CHI@Ins-NPs) 的制造,装有胰岛素纳米粒子 (Ins-NPs).
- 通过人工粘素和体外药物输送/保留在隔离的小肠中的微运动透的评估.
- 使用UPLC-MS确定胰岛素,分析胰岛素离子的质量电荷比.
主要成果:
- 基于Mg的微电机成功地装载了Ins-NPs,并证明了通过人工粘素的透.
- 与惰性颗粒相比,微电机在隔离的小肠中增强了胰岛素的保留.
- 特定的胰岛素离子 ([胰岛素+6H]6+,[胰岛素+5H]5+,[胰岛素+4H]4+) 被UPLC-MS检测到,仅在微运动系统中检测到.
- 基于Mg的微电机系统显示出良好的生物相容性,并简化了UPLC-MS的样本准备.
结论:
- 基于Mg的微电机提供了一种有前途的策略,用于有效的肠道药物输送,克服生物障碍.
- 这种方法显著增强了药物保留,并通过UPLC-MS. 能够灵敏地检测低药物含量.
- 这项研究扩大了在制药分析和诊断中无微电机的传感应用.
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