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基酸可以缓解甲基酸诱导的卵细胞功能障碍,在小鼠种类的体外成熟过程中缓解

Shahrzad Ronasi¹, Amir Hossein Mahdavi¹, Shiva Rouhollahi Varnosfaderani²

¹Department of Animal Science, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.

|March 25, 2025

概括

基酸 (PA) 通过恢复氧化还原平衡和改善胚胎发育,防止甲基酸 (MGO) 诱导的卵细胞功能障碍. 这种抗氧化剂可能会改善肥胖和糖尿病等疾病的生殖健康.

科学领域:

生殖生物学生殖生物学
氧化压力研究研究氧化压力研究
营养科学营养科学

背景情况:

由反应性代谢物和先进的糖化最终产品 (AGEs) 标志着二碳醇应激,有助于肥胖,糖尿病和生殖问题.
甲基醇 (MGO) 是一种关键的二碳尼尔代谢物,会降低卵细胞质量和发育潜力.
来自石榴种子油的抗氧化剂基酸 (PA) 正在研究其保护作用.

研究的目的:

研究基酸 (PA) 对甲基酸 (MGO) 诱导的卵细胞功能障碍的保护作用.
评估PA对MGO压力下的卵细胞成熟,胚胎发育和分子通路的影响.

主要方法:

暴露于MGO (75μM) 的卵细胞的体外成熟,有或没有PA补充.
评估卵细胞成熟率,前核形成和胚胎细胞发育.
对氧化还原状态 (ROS,GSH),线粒体分布和基因表达 (Glo1,Rage,Nrf2,Nf-κB) 的分析.

主要成果:

暴露于MGO减少了卵细胞成熟和胚胎发育率.
补充PA部分扭转了MGO的不良影响,改善了成熟率和胚胎囊率.
PA恢复了氧化还原平衡,维护了线粒体分布,并调节了与二碳烯应激和氧化反应相关的关键基因表达.

结论:

普尼西克酸显示出对MGO诱导的卵细胞功能障碍的保护作用.
通过减轻氧化应激和二碳酸损伤,PA提高了卵细胞质量和植入前胚胎发育.
在与二乙烯压力相关的条件下,PA显示出作为改善生殖健康的饮食干预的潜力.

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