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Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
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特里斯通过线性混合抑制机制抑制GH1β-葡萄糖酶

Rafael S Chagas1, Sandro R Marana1

  • 1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP, Brazil.

PloS one
|March 25, 2025
PubMed
概括

一种常见的缓冲剂Tris作为Spodoptera frugiperda GH1β-glucosidase (Sfβgly) 的混合抑制剂. 这种缓冲器可以降低酶活性,并导致不准确的动力参数测量.

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