相关实验视频

Updated: May 20, 2025

Induction and Testing of Hypoxia in Cell Culture

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

低压性缺氧暴露会通过HIF-1α信号通路损害结肠小杯细胞亚群

Shaojie Zhang^1,2, Xiufang Jiang³, Wangjingyi Zhang²

¹Department of Gastroenterology, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, Beijing, People's Republic of China.

American journal of physiology. Gastrointestinal and liver physiology

|March 25, 2025

概括

由于高海拔暴露而导致的低巴性缺氧会减少结肠杯细胞和粘液分泌. 缺氧诱导因子-1α (HIF-1α) 通过缺氧激活会损害小杯细胞功能和肠壁完整性.

科学领域:

胃肠病学胃肠病学
高度生理学高度生理学
细胞生物学细胞生物学

背景情况:

高空暴露会导致低氧,影响肠道屏障功能.
玻璃杯细胞对于肠道屏障完整性至关重要,但它们对缺氧的反应尚不清楚.

研究的目的:

为了研究催性缺氧对结肠杯细胞的影响.
阐明分子机制,特别是缺氧诱导因子-1α (HIF-1α) 在缺氧诱导的杯状细胞变化中的作用.

主要方法:

建立了一个小鼠模型的低压性缺氧 (6,000 m).
利用经过分类的结肠杯细胞的转录组分析.
采用了缺氧治疗的杯状细胞系 (Ls174t) 和siRNA用于HIF-1α敲击.

主要成果:

低血压缺氧使结肠杯细胞数量减少了27.6%,并影响了粘液分泌.
观察到杯状细胞标记基因表达 (例如,CLCA1,FCGBP) 的显著变化.
HIF-1α激活降低了正规杯状细胞标志物表达,而HIF-1α敲击增加了它.

结论:

低血压缺氧会对结肠杯细胞数量和功能产生负面影响.
HIF-1α信号通路调解了缺氧诱导的杯状细胞变化.
准HIF-1α可能提供一种策略,在低氧条件下恢复杯状细胞功能和肠壁完整性.

关键词:

这就是HIF-1α.结肠杯细胞亚种群结肠杯细胞亚种群催眠症的催眠症暴露暴露暴露.肠道粘膜屏障肠道粘膜屏障肠道粘膜屏障翻译学翻译学翻译学翻译学

更多相关视频

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

相关概念视频

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...