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Updated: May 20, 2025

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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基于芯片的数字PCR与大场一次性成像,用于高灵敏度核酸量化

Jinrong Shen1, Jingxing Fan1, Zhehao Zhao2

  • 1State Key Laboratory of ASIC and System, Fudan University, Shanghai, 200433, China; School of Microelectronics, Fudan University, Shanghai, 200433, China.

Biosensors & bioelectronics
|March 25, 2025
PubMed
概括

我们开发了一种基于芯片的数字聚合酶连锁反应 (cdPCR) 装置,配备了一种新的大场成像系统. 该系统能够快速,一次性获取图像,改善dPCR分析中的绝对量化.

关键词:
绝对量化的绝对量化.基于芯片的数字PCR.光图像分析 光图像分析高均性的照明系统.肺癌的DNA 肺癌的DNA一次性成像成像技术

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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