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miR-200b-3p通过与CircADGRF5竞争来调节SESN1表达来影响前脂肪细胞的增殖和分化

Pengtao Yuan¹, Hongtai Li¹, Hongyuan Zhang¹

¹The Shennong Laboratory, Henan Agricultural University, Zheng Zhou, Henan Province, 450046, PR China.

Poultry science

|March 25, 2025

概括

微RNAs调节的脂肪发育. 具体来说,miR-200b-3p针对SESN1控制脂肪细胞的增殖和分化,而circADGRF5则影响这种相互作用.

科学领域:

分子生物学分子生物学
动物科学动物科学
遗传学遗传学是一个

背景情况:

的过度腹部脂肪影响了家禽业.
非编码RNA因其在脂肪发育中的作用而越来越受认可.
miR-200b-3p以前被确定在的脂肪发育过程中被差异地表达.

研究的目的:

研究miR-200b-3p在前脂肪细胞增殖和分化中的作用.
为了确定miR-200b-3p在脂肪生成中的分子标和调节网络.
在这个过程中验证新型circRNA,circADGRF5的功能.

主要方法:

RNA测序 (RNA-seq) 分析分析
路西法雷斯记者测量以确认直接结合.
对miR-200b-3p和SESN1的过度表达和抑制研究.
验证circRNA表达和功能功能的验证

主要成果:

miR-200b-3p直接准SESN1的3'UTR,促进前脂肪细胞的增殖和抑制分化.
过度表达SESN1会抑制增殖并促进分化,而抑制则会产生相反的效果.
一种新的circRNA,circADGRF5,被确定并被证明通过与miR-200b-3p竞争性结合来调节SESN1表达,形成一个ceRNA网络.

结论:

miR-200b-3p通过准SESN1.1,直接调节前脂肪细胞的增殖和分化.
该circADGRF5/miR-200b-3p/SESN1 ceRNA网络在前脂肪细胞发育中发挥作用.
这些发现为控制的脂肪沉积的分子机制提供了洞察力.

关键词:

在SESN1;预脂细胞.网络 ceRNA 网络一个圆周的GRF5ADGRF5 在 miR-200b-3p 中使用.

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