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相关概念视频

Microbial Fermentation01:23

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
Bioreactor Controls-I01:28

Bioreactor Controls-I

Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

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相关实验视频

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Using Coculture to Detect Chemically Mediated Interspecies Interactions
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Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

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使用网络控制指导微生物共同培养成分.

Ting An Lee1, Jan Morlock2, John Allan1

  • 1Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK.

Cell reports methods
|March 25, 2025
PubMed
概括

这项研究介绍了一种控制微生物共同培养的网络方法,而不需要基因工程. 该方法使用生物反应器数据和温度调整来精确管理细菌种群,为合成生物学提供了广泛的应用.

关键词:
CP:生物技术 生物技术培训班:微生物学检查人员控制PI的控制生物膜是一种生物膜.生物反应器是一个生物反应器.共同文化是一种共同文化.控制 控制 控制 控制网络遗传学 网络遗传学网络网络技术 网络网络系统生物学 系统生物学

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Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria
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Assembly and Quantification of Co-Cultures Combining Heterotrophic Yeast with Phototrophic Sugar-Secreting Cyanobacteria

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科学领域:

  • 合成生物学 合成生物学
  • 生物技术是生物技术.
  • 控制工程 控制工程 控制工程

背景情况:

  • 微生物共同培养在生物技术中至关重要,但难以控制.
  • 现有的方法通常依赖于基因工程,限制了广泛的适用性.
  • 需要精确的,非侵入性的控制策略来稳定共同培养管理.

研究的目的:

  • 开发和演示一种控制细菌共同培养 (Pseudomonas putida和Escherichia coli) 组成的网络框架.
  • 为了实现这种控制,不需要使用基因工程来实现细胞-计算机接口.
  • 建立一种广泛适用的方法,以稳定管理多样化的微生物联盟.

主要方法:

  • 利用生物反应器测量来提取实时成分信息.
  • 集成测量与系统模型使用扩展卡尔曼波器用于准确的状态估计.
  • 使用温度作为控制输入,利用不同物种的最佳生长温度.
  • 实施了一种比例积分 (PI) 控制算法,用于动态参考跟踪和噪声排斥.

主要成果:

  • 从杂的生物反应器数据中成功估计了共同培养成分.
  • 已经证明了微生物组成的温度控制.
  • 使用PI控制实现了稳定的共同培养维持7天 (约250代).
  • 从初始注射比率和有效的实时噪声排斥中展示了独立性.

结论:

  • 开发的网络网络框架使微生物共同培养的精确,非遗传控制成为可能.
  • 这种方法为合成生物学和生物技术中的微生物联盟管理提供了一个强大而广泛适用的战略.
  • 该方法成功稳定了P. putida和E. coli的共同培养,证明了其实际可行性.