建立了尼古丁胺胺单核酸生物合成的新途径
Rongchen Feng1, Ziting Yan1, Guoguang Wei2
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu 211816, China.
Enzyme and microbial technology
|March 25, 2025
概括
研究人员为尼古丁胺单核酸 (NMN) 生物合成开发了新的NRK依赖性途径. 这项研究为NMN提供了一种可行的生物生产方法,NMN是代谢健康的关键NAD+前体.
科学领域:
- 生物化学 生物化学
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 尼古丁胺胺单核酸 (NMN) 对于细胞能量代谢是必不可少的,作为尼古丁胺胺氨基二核酸 (NAD+) 的前体.
- 对生物NMN生产方法的兴趣正在增加.
- 现有的NMN生产方法可能有局限性.
研究的目的:
- 为生物NMN生产开发新的NRK依赖的合成路径.
- 优化酶级联以实现高效的NMN生物合成.
- 为了在大肠杆菌中实现高位数NMN的产生.
主要方法:
- 设计了两种D-ribose-1-phosphate (R-1-P) 供应策略:外部D-ribose酸化和从葡萄糖转化酸路径.
- 优化了体外多酶级联 (XapA/PNP/NRK,PPM,NRK) 以确定最有效的NMN生物合成催化剂.
- 通过过度表达布,淘汰竞争性途径,并使用PnuC转运器增强分泌物来改造大肠杆菌.
主要成果:
- 确定NRK是从D-ribose和niacinamide中NMN生物合成的最有效的催化剂.
- 在工程Escherichia coli中达到62.0 mg/L的NMN标位.
- 证明了NMN的成功生物生产.
结论:
- 开发的NRK-依赖的合成途径为NMN生物合成提供了可行的替代方案.
- 这项研究推进了生物NMN生产领域.
- 工程化的大肠杆菌菌株为可扩展的NMN制造提供了一个平台.
关键词:
基因编辑CRISPR-Cas9基因编辑尼古丁胺胺单核酸是什么尼古丁胺胺里波斯 (Nicotinamide ribose) 是一种尼古丁胺的化合物.基酸酶酶的使用方法信号的信号.运输蛋白质是运输蛋白质的一种.更多相关视频
相关概念视频
Role of Reduced Coenzymes NADH and FADH₂
11.1K
The energy released from the breakdown of the chemical bonds within nutrients can be stored either through the reduction of electron carriers or in the bonds of adenosine triphosphate (ATP). In living systems, a small class of compounds functions as mobile electron carriers, molecules that bind to and shuttle high-energy electrons between compounds in pathways. The principal electron carriers that will be considered originate from the B vitamin group and are derivatives of nucleotides; they are...
11.1K
Overview of Nitrogen Metabolism
7.8K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of ammonia, ammonium ions, nitrate, nitrite, or nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
7.8K
Nitric Oxide Signaling Pathway
4.9K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
4.9K
2° Amines to N-Nitrosamines: Reaction with NaNO2
4.0K
Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
4.0K
ATP and Macromolecule Synthesis
5.2K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
5.2K
Nucleic Acids and Nucleotides
8.6K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and have instructions for its functioning. The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria....
Deoxyribonucleic Acid (DNA)
DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and the organelles such as chloroplasts and mitochondria....
8.6K


