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Updated: May 20, 2025

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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康登森II在S阶段与BLM酶相互作用,以保持基因组稳定性

Brian Rodemoyer1, Ganesha Kariyawasam1, Veena Subramanian1

  • 1Department of Molecular Biosciences, University of South Florida, 4202 E. Fowler Ave., Tampa, FL, 33620, USA.

Communications biology
|March 26, 2025
PubMed
概括

康登森II蛋白与布鲁姆综合征酶 (BLM) 相互作用,以保持基因组的稳定性. 这种相互作用对于DNA复制,修复和预防细胞周期期间的基因组不稳定性至关重要.

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Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
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Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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