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在QCM金电极上的PEI层中有效地固定hnnRNPA2B1蛋白

Olga Volkova1, Viacheslav Kravtsov1, Ekaterina V Skorb1

  • 1Infochemistry Scientific Center, ITMO University, Saint Petersburg 191002, Russia.

Langmuir : the ACS journal of surfaces and colloids
|March 26, 2025
PubMed
概括

这项研究优化了使用聚乙烯胺 (PEI) 层在黄金电极上固定异质核核核蛋白A2B1 (hnRNPA2B1). 在RBP研究中,线性PEI和增加的离子强度显著改善了hnRNPA2B1吸附.

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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

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Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...