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相关概念视频

RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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相关实验视频

Updated: Jun 16, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

IPANEMAP套件:用于探测信息的RNA结构建模的管道.

Pierre Hardouin1, Nan Pan2, Francois-Xavier Lyonnet du Moutier1

  • 1CNRS UMR 8038, CiTCoM Cibles Thérapeutiques et Conception de Médicaments, Université Paris Cité, 4 avenue de l'Observatoire, 75270 Paris, France.

NAR genomics and bioinformatics
|March 26, 2025
PubMed
概括
此摘要是机器生成的。

IPANEMAP套件简化了RNA结构分析,从化学探测数据到二级结构模型. 这个平台集成了实验数据集,用于准确的RNA结构建模和比较分析.

更多相关视频

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

相关实验视频

Last Updated: Jun 16, 2026

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • RNA结构对于功能至关重要,但很难通过实验来确定.
  • 计算式RNA结构建模从整合实验数据 (如化学探测) 中获益.
  • 目前对RNA结构探测数据的分析通常是复杂的,需要人工干预.

研究的目的:

  • 介绍IPANEMAP套件,这是一个简化RNA二次结构建模的平台.
  • 为了提高准确性,促进多种化学探测数据集的整合.
  • 为了使在各种条件和相互作用下进行比较RNA结构分析.

主要方法:

  • 开发IPANEMAP套件平台的开发工作.
  • 低分辨率实验数据 (化学探测) 与计算建模的整合.
  • 使用集成建模与多种化学探测数据集和植物遗传数据.

主要成果:

  • IPANEMAP Suite成功地引导用户从原始化学探测数据到信息化的二次结构模型.
  • 该平台可以无集成各种实验数据集进行比较分析.
  • 在IPANEMAP套件中的整合建模显著提高了RNA二级结构模型的准确性.

结论:

  • IPANEMAP套件为RNA结构分析提供了一个全面且易于使用的解决方案.
  • 该平台提高了RNA二次结构建模的准确性和可追溯性.
  • IPANEMAP套件简化了RNA结构的研究,包括与蛋白质和小分子的相互作用.