相关实验视频
Updated: May 20, 2025
12:31
In Vivo Modeling of the Morbid Human Genome using Danio rerio
Published on: August 24, 2013
20.6K
在tigecycline的选择性压力下,在体外适应性实验室进化过程中,Staphylococcus aureus的表型和遗传阶段性变化
Honghao Huang1,2, Peng Wan1,2, Yiyi Chen3
1National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, Guangdong, China.
Antimicrobial agents and chemotherapy
|March 26, 2025
概括
使用适应性实验室进化,开发了高水平的抗tigecycline的金黄色葡萄球菌突变菌. 鉴定出 yycH 基因对于降低这些抗性金黄色细菌菌株的tigecycline 敏感性至关重要.
科学领域:
- 微生物学 微生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 提格环素耐药性在金黄色葡萄球菌感染中越来越令人担忧.
- 适应性实验室进化 (ALE) 已被用于研究细菌的耐药性机制.
- 之前针对tigecycline耐药性的ALE研究一直专注于格拉姆阴性细菌.
研究的目的:
- 为了开发高水平的对tigecycline耐药的金黄色葡萄球菌突变 (TRSAms).
- 调查S. aureus中降低tigecycline敏感性的潜在机制.
- 为了确定与tigecycline耐药性相关的遗传突变.
主要方法:
- 连续增加的tigecycline度被用于适应性实验室进化.
- 通过增长率,自解,突变频率和毒性测试来评估表型变化.
- 进行了全基因组测序和克隆实验,以确定致病突变.
主要成果:
- 成功地产生了高水平的抗环素的金黄色细菌突变 (TRSAms).
- 在高水平TRSAms中多次观察到yycH和fakA的同步突变.
- yycH基因的补充恢复了tigecycline的敏感性,证实了它的关键作用.
结论:
- yycH基因在降低Staphylococcus aureus的tigecycline敏感性方面发挥着至关重要的作用.
- ALE是一种有效的方法来产生耐药突变并阐明耐药性机制.
- 在yycH和rpsJ中的突变与S. aureus中的tigecycline耐药性有关.
相关概念视频
02:07Tumor Progression
6.2K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.2K
01:43Incomplete Dominance
20.6K
Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.
20.6K
02:27Background and Environment Affect Phenotype
6.4K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
6.4K
02:46Genetic Screens
4.8K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
4.8K
01:11Genetic Lingo
98.8K
Overview
98.8K
01:35Pedigree Analysis
83.2K
Overview
83.2K