高能增强玉米对Holotrichia parallela的敏感性,通过β-Caryophyllene介导的嗅觉识别和雅斯莫纳特抑制
Shiwen Zhao1,2, Xiao Wang1,3, Zhun Wang4
1College of Plant Science, Jilin University, Changchun 130062, P. R. China.
Journal of agricultural and food chemistry
|March 26, 2025
概括
化肥改变了玉米的新陈代谢,影响了害虫的相互作用. 高吸引了特定化合物的害虫,而低触发了植物防御,影响了害虫管理策略.
科学领域:
- 农业科学 农业科学
- 植物新陈代谢 植物新陈代谢
- 昆虫与植物的相互作用
背景情况:
- 肥对于作物产量至关重要,但可以改变植物害虫的动态.
- 玉米 (Zea mays) 易受害虫的影响,比如Holotrichia parallela,这是一种生活在土壤中的昆虫.
- 了解如何影响玉米的化学防御和吸引力,对于可持续农业至关重要.
研究的目的:
- 研究化肥水平对玉米新陈代谢的影响.
- 为了确定改变玉米新陈代谢如何影响害虫Holotrichia parallela的行为.
- 阐明特定挥发性有机化合物 (VOC) 在调解植物-害虫相互作用中的作用.
主要方法:
- 在低 (LN) 和高 (HN) 条件下对玉米代谢的比较分析.
- 气色谱-质谱 (GC-MS) 用于识别玉米排放的VOC.
- 行为测试用于评估害虫对特定VOC的吸引力.
- RNA干扰 (RNAi) 来确认嗅觉受体基因在害虫反应中的功能.
主要成果:
- HN条件增加了VOC排放,β-caryophyllene和棕酸吸引了害虫幼虫,β-caryophyllene吸引了雌性卵巢.
- 针对HparOR19和HparOR22的RNAi证实了β-caryophyllene在害虫嗅觉识别中的作用.
- LN条件增强了与莉酸 (JA) 相关的防御通路.
- HN条件促进了主要代谢物 (葡萄糖,果糖,糖糖),刺激了害虫的食.
结论:
- 化肥显著重塑了玉米的代谢特征及其与害虫的相互作用.
- 在HN条件下,特定的VOC作为吸引H的吸引剂. 平行,而LN条件主要的植物防御.
- 将代谢洞察纳入管理是有效的害虫控制策略的关键.
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